CAJ | 학술논문

目的：探讨细胞内Zn2＋的浓度在低氧调节髓核细胞表达金属蛋白酶（metalloproteinases ，MMPs）和细胞外基质（ECM）中的作用及其机制。方法：从SD大鼠中提取髓核细胞后首先进行平面培养，然后用藻酸钠凝胶进行三维培养。采用Fluo‐Zin‐3 AM染色法检测细胞内Zn2＋的浓度（intracellular Zn2＋concentration ，[Zn2＋]i）；采用阿利新蓝染色分析细胞分泌蛋白多糖的含量，采用二甲基亚甲蓝分光光度法（DMMB）检测糖胺聚糖的表达水平，采用real‐time PCR分析II型胶原（α1 type II collagen ，COL2A1）、金属蛋白酶13（matrix metalloproteinase 13，MMP‐13）和解整合素样金属蛋白酶5（a disintegrin and met‐alloproteinase with a thrombospondin motif 5，ADAMTS‐5）mRNA的表达。通过免疫组化法和Western印迹法分析锌铁转运蛋白8（ZRT ，IRT‐like protein 8，ZIP8）的表达水平。结果：IL‐1β和ZnCl2可以显著提高髓核细胞内的[Zn2＋]i ，但是该作用可以被低氧所抑制。在藻酸钠三维培养的髓核细胞中，低氧可以显著改善 IL‐1β和 ZnCl2引起的蛋白多糖、糖胺聚糖和COL2A1 mRNA的降低，但ZnCl2可以抑制低氧的保护作用。细胞内Zn2＋的螯合剂和低氧可以抑制MMP‐13 mRNA水平的升高。IL‐1β和ZnCl2促进髓核细胞中ZIP8的表达升高，但是低氧可抑制ZIP8的表达。结论：低氧可以调节髓核细胞中Zn2＋的内流，Zn2＋介导了低氧对髓核细胞中M M P‐13和ECM的调节作用。[Zn2＋]i的变化可能参与了椎间盘退变的过程。
목적：탐토세포내Zn2＋적농도재저양조절수핵세포표체금속단백매（metalloproteinases ，MMPs）화세포외기질（ECM）중적작용급기궤제。방법：종SD대서중제취수핵세포후수선진행평면배양，연후용조산납응효진행삼유배양。채용Fluo‐Zin‐3 AM염색법검측세포내Zn2＋적농도（intracellular Zn2＋concentration ，[Zn2＋]i）；채용아리신람염색분석세포분비단백다당적함량，채용이갑기아갑람분광광도법（DMMB）검측당알취당적표체수평，채용real‐time PCR분석II형효원（α1 type II collagen ，COL2A1）、금속단백매13（matrix metalloproteinase 13，MMP‐13）화해정합소양금속단백매5（a disintegrin and met‐alloproteinase with a thrombospondin motif 5，ADAMTS‐5）mRNA적표체。통과면역조화법화Western인적법분석자철전운단백8（ZRT ，IRT‐like protein 8，ZIP8）적표체수평。결과：IL‐1β화ZnCl2가이현저제고수핵세포내적[Zn2＋]i ，단시해작용가이피저양소억제。재조산납삼유배양적수핵세포중，저양가이현저개선 IL‐1β화 ZnCl2인기적단백다당、당알취당화COL2A1 mRNA적강저，단ZnCl2가이억제저양적보호작용。세포내Zn2＋적오합제화저양가이억제MMP‐13 mRNA수평적승고。IL‐1β화ZnCl2촉진수핵세포중ZIP8적표체승고，단시저양가억제ZIP8적표체。결론：저양가이조절수핵세포중Zn2＋적내류，Zn2＋개도료저양대수핵세포중M M P‐13화ECM적조절작용。[Zn2＋]i적변화가능삼여료추간반퇴변적과정。
Objective:To explore the effect and mechanism of intracellular Zn2+ concentration ([Zn2+ ]i) in hypoxia‐induced regulation of metalloproteinases (MMPs) and extracellular matrix (ECM) expression in nucleus pulposus (NP) cells .Methods:NP cells from SD rats received plate culture at first and then three‐dimensional culture with sodium alginate gel .[Zn2+ ]i was assayed by FluoZin‐3 AM staining .Proteoglycan was assayed by Alcian blue staining .Glycosaminoglycan was detected by 1 ,9‐dimethylmethylene blue (DMMB) assay .And real‐time PCR were used to assay the mRNA expression of α1 type II collagen (COL2A1) ,matrix metalloproteinase 13 (MMP‐13) and a disintegrin and metalloproteinase with a thrombospondin motif 5 (ADAMTS‐5) .The expression of ZRT ,IRT‐like protein 8(ZIP8) was assayed by immunohistochemistry and Western blotting .Results:Interleukin (IL)‐1βand ZnCl2 could significantly increase the [Zn2+ ]i of NP cells ,however ,the effect could be inhibited by hypoxia .Hypoxia did significantly attenuate the decrease of proteoglycan ,glycosaminoglycan ,and COL2A1 mRNA ,which was induced by IL‐1βand ZnCl2 treatment ,in sodium alginate three‐dimensional culture . However ,ZnCl2 inhibited the protective effect of hypoxia .Both an intracellular Zn2+chelator and hypoxia could inhibit the increase of MMP‐13 mRNA expression .IL‐1βand ZnCl2 treatment promoted the increase of ZIP8 expression in NP cells ,however ,hypoxia inhibited ZIP8 expression .Conclusions:Hypoxia may regulate the Zn2+ influx in NP cells . Zn2+ mediates the regulation effect of hypoxia on ECM and MMP‐13 .Perhaps the changes of [Zn2+ ]i are involved in the process of intervertebral disc degeneration .