南京林业大学学报（自然科学版）
南京林業大學學報（自然科學版）
남경임업대학학보（자연과학판）
Journal of Nanjing Forestry University (Natural Science Edition)
2015年
6期
155-162
,共8页
耗散型石英晶体微天平%纤维素%生物传感器%吸附与解吸%酶水解动力学
耗散型石英晶體微天平%纖維素%生物傳感器%吸附與解吸%酶水解動力學
모산형석영정체미천평%섬유소%생물전감기%흡부여해흡%매수해동역학
QCM-D%cellulose%biosensor%adsorption and desorption%kinetics of enzymatic hydrolysis
酶水解包含酶的吸附、解吸及其对纤维素的降解过程，是纤维素生物转化的重要途径。耗散型石英晶体微天平（ QCM－D）是基于石英的压电特性制备的一种表面敏感型分析技术，能感应到纳克级的质量变化。不同于传统的化学方法，QCM－D可实时反映其动态过程及动力学特征。笔者概述了QCM－D的原理、生物传感器的成熟制备方式及其在纤维素酶水解方面的应用研究成果。分析认为，通过QCM－D在线测定酶水解过程中频率和能量耗散变化，可原位、实时响应水解底物表面的质量变化，直观地反映纤维素酶在底物上的吸附、解吸，以及纤维素酶水解的动态过程。由QCM－D呈现的频率和耗散间的关系还可间接获得吸附层的结构信息。但QCM－D用于酶水解时也存在一定的局限性，这一方法对底物的成膜性要求较高，在定量分析方面也有待深入研究。
酶水解包含酶的吸附、解吸及其對纖維素的降解過程，是纖維素生物轉化的重要途徑。耗散型石英晶體微天平（ QCM－D）是基于石英的壓電特性製備的一種錶麵敏感型分析技術，能感應到納剋級的質量變化。不同于傳統的化學方法，QCM－D可實時反映其動態過程及動力學特徵。筆者概述瞭QCM－D的原理、生物傳感器的成熟製備方式及其在纖維素酶水解方麵的應用研究成果。分析認為，通過QCM－D在線測定酶水解過程中頻率和能量耗散變化，可原位、實時響應水解底物錶麵的質量變化，直觀地反映纖維素酶在底物上的吸附、解吸，以及纖維素酶水解的動態過程。由QCM－D呈現的頻率和耗散間的關繫還可間接穫得吸附層的結構信息。但QCM－D用于酶水解時也存在一定的跼限性，這一方法對底物的成膜性要求較高，在定量分析方麵也有待深入研究。
매수해포함매적흡부、해흡급기대섬유소적강해과정，시섬유소생물전화적중요도경。모산형석영정체미천평（ QCM－D）시기우석영적압전특성제비적일충표면민감형분석기술，능감응도납극급적질량변화。불동우전통적화학방법，QCM－D가실시반영기동태과정급동역학특정。필자개술료QCM－D적원리、생물전감기적성숙제비방식급기재섬유소매수해방면적응용연구성과。분석인위，통과QCM－D재선측정매수해과정중빈솔화능량모산변화，가원위、실시향응수해저물표면적질량변화，직관지반영섬유소매재저물상적흡부、해흡，이급섬유소매수해적동태과정。유QCM－D정현적빈솔화모산간적관계환가간접획득흡부층적결구신식。단QCM－D용우매수해시야존재일정적국한성，저일방법대저물적성막성요구교고，재정량분석방면야유대심입연구。
The adsorption and desorption of enzymes on the substrates are critical for bioconversion of cellulosic materi?als. QCM?D ( quartz crystal microbalance with dissipation) , different from traditional chemical analysis methods, could reflect the whole dynamic process and the features of kinetics of enzymatic hydrolysis online. QCM?D was a surface?sensi?tive analytical technique based on the piezoelectric properties of quartz crystal and could sense the mass change in nanoscale. In this review, the operating principle of QCM?D and the preparation methods of the biosensor, as well as the latest developments of the application of QCM?D on the research of enzymatic hydrolysis of cellulosic materials were in?troduced. Generally, QCM?D gives the information in situ and responses to the surface quality changes on the substrates on top of quartz crystal in real?time through the variations of frequency and dissipation. Thus the adsorption and desorp?tion of enzymes on the substrate, and the dynamic enzymatic hydrolysis could be visually monitored and analyzed. In ad?dition, the relationships between frequency and dissipation obtained from QCM?D reflect the structure information of ad?sorbed layers. However, there still were part of limitations in enzymatic hydrolysis process. For instance, relatively high demands of film?formation of substrates were needed when using QCM?D. Besides, further researches were required in the aspect of quantitative analysis.
