CAJ | 학술논문

为了对进境口岸截获的俄罗斯木材中的墨天牛属（非中国种）害虫进行准确、快速鉴定，笔者利用DNA 条码试剂盒检测技术，基于墨天牛线粒体COⅠ（线粒体细胞色素氧化酶亚基I）基因设计一对巢式引物，对截获墨天牛属中的8个种进行COⅠ扩增，将扩增序列上传至有害生物信息网站，依据碱基位点在不同种间的排列和组成的差异，获得不同种在相应位点上的特有碱基，即标记性碱基，以此作为区分墨天牛种的依据。通过这种墨天牛属DNA 条码试剂盒检测技术，可为口岸检疫鉴定提供有效的技术手段。
위료대진경구안절획적아라사목재중적묵천우속（비중국충）해충진행준학、쾌속감정，필자이용DNA 조마시제합검측기술，기우묵천우선립체COⅠ（선립체세포색소양화매아기I）기인설계일대소식인물，대절획묵천우속중적8개충진행COⅠ확증，장확증서렬상전지유해생물신식망참，의거감기위점재불동충간적배렬화조성적차이，획득불동충재상응위점상적특유감기，즉표기성감기，이차작위구분묵천우충적의거。통과저충묵천우속DNA 조마시제합검측기술，가위구안검역감정제공유효적기술수단。
In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene ( mitochondrial cyto?chrome oxidase subunit I) . Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and up?loaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.