农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
Agricultural Science & Technology
2015年
10期
2179-2182,2317
,共5页
水稻%稻曲病%致病菌株%抗病性%数量性状位点
水稻%稻麯病%緻病菌株%抗病性%數量性狀位點
수도%도곡병%치병균주%항병성%수량성상위점
Rice%Rice false smut%Pathogen strains%Disease resistance%Quantita-tive trait locus
为了鉴定水稻对稻曲病的抗病基因，利用157个家系组成的大关稻（ japonica）/IR28（indica）重组自交系（Recombinant inbred lines, RIL）群体，采用人工接种方法，以发病病情指数作为表型值。2012和2013年，接种鉴定亲本及RILs对水稻稻曲病致病菌株 Pi-1的抗性。利用 QTL Cartographer 分析软件，对水稻抗 Pi-1菌株基因进行检测分析。两年共检测到7个QTL，分别为位于第2、7、8、11和12染色体上的 qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12，单个位点的贡献率介于8.5%~17.2%之间。其中，2012年检测到qFsr2a、qFsr2b、qFsr8a、qFsr11等4个位点；2013年检测到 qFsr7、qFsr8b、qFsr11、qFsr12等4个位点。 qFsr11在两年中均被检测到，对性状的解释率为13.5%和17.2%，作用效应使病情指数下降9.9%和14.3%，提高了抗病性。根据抗性位点加性效应方向，qFsr2a、qFsr8a、qFsr8b、qFsr11和 qFsr12等位点是来自于亲本IR28的增效等位基因，而位点 qFsr2b和 qFsr7的抗性效应方向相反，是来自于大关稻。稳定遗传的抗病位点 qFsr11及其紧密连锁的分子标记可以在分子标记辅助选择育种中得以应用。
為瞭鑒定水稻對稻麯病的抗病基因，利用157箇傢繫組成的大關稻（ japonica）/IR28（indica）重組自交繫（Recombinant inbred lines, RIL）群體，採用人工接種方法，以髮病病情指數作為錶型值。2012和2013年，接種鑒定親本及RILs對水稻稻麯病緻病菌株 Pi-1的抗性。利用 QTL Cartographer 分析軟件，對水稻抗 Pi-1菌株基因進行檢測分析。兩年共檢測到7箇QTL，分彆為位于第2、7、8、11和12染色體上的 qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12，單箇位點的貢獻率介于8.5%~17.2%之間。其中，2012年檢測到qFsr2a、qFsr2b、qFsr8a、qFsr11等4箇位點；2013年檢測到 qFsr7、qFsr8b、qFsr11、qFsr12等4箇位點。 qFsr11在兩年中均被檢測到，對性狀的解釋率為13.5%和17.2%，作用效應使病情指數下降9.9%和14.3%，提高瞭抗病性。根據抗性位點加性效應方嚮，qFsr2a、qFsr8a、qFsr8b、qFsr11和 qFsr12等位點是來自于親本IR28的增效等位基因，而位點 qFsr2b和 qFsr7的抗性效應方嚮相反，是來自于大關稻。穩定遺傳的抗病位點 qFsr11及其緊密連鎖的分子標記可以在分子標記輔助選擇育種中得以應用。
위료감정수도대도곡병적항병기인，이용157개가계조성적대관도（ japonica）/IR28（indica）중조자교계（Recombinant inbred lines, RIL）군체，채용인공접충방법，이발병병정지수작위표형치。2012화2013년，접충감정친본급RILs대수도도곡병치병균주 Pi-1적항성。이용 QTL Cartographer 분석연건，대수도항 Pi-1균주기인진행검측분석。량년공검측도7개QTL，분별위위우제2、7、8、11화12염색체상적 qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12，단개위점적공헌솔개우8.5%~17.2%지간。기중，2012년검측도qFsr2a、qFsr2b、qFsr8a、qFsr11등4개위점；2013년검측도 qFsr7、qFsr8b、qFsr11、qFsr12등4개위점。 qFsr11재량년중균피검측도，대성상적해석솔위13.5%화17.2%，작용효응사병정지수하강9.9%화14.3%，제고료항병성。근거항성위점가성효응방향，qFsr2a、qFsr8a、qFsr8b、qFsr11화 qFsr12등위점시래자우친본IR28적증효등위기인，이위점 qFsr2b화 qFsr7적항성효응방향상반，시래자우대관도。은정유전적항병위점 qFsr11급기긴밀련쇄적분자표기가이재분자표기보조선택육충중득이응용。
In order to identify the resistant gene of rice false smut in rice, a recombi-nant inbred line (RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao/IR28 by the single seed descent method was used to detect quantitative trait loci (QTLs) conferring resistance to strain Pi-1 of rice false smut caused by Ustilaginoidea virens (Cooke) Takahashi in 2012 and 2013. The disease rate indexes of the two parents and 157 RILs caused by the strain Pi-1 of rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software. Seven QTLs control ing false smut re-sistance were detected on chromosomes 2, 7, 8, 11 and 12, respectively, with the phenotypic variance of 8.5%-17.2%. There were four QTLs detected in 2012 and 2013, respectively, and only one QTL was found in both two years, the phenotypic variation explained by this individual QTL was 13.5% and 17.2%, and the additive effects of this QTL contributed to the 9.9% and 14.3% decrease of disease index and therefore the disease resistance increased. The direction of the additive effects at five loci qFsr2a, qFsr8a, qFsr8b, qFsr11 and qFsr12 coincided with that predicted by phenotypes of the parents, and the IR28 al eles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao al eles at qFsr2b and qFsr7. The qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection (MAS) program.
