CAJ | 학술논문

利用国内公开发表的西瓜抗枯萎病生理小种1基因紧密连锁标记4451_fon，首次对我们抗枯萎病育种选出的二倍体和四倍体种质进行了分子鉴定，结果表明，全部二倍体单系和四倍体单系均表现为抗病基因型，判定为抗病材料，与人工接种鉴定结果完全一致；分子鉴定还表明，这些抗病单系为抗病纯合体，在以后的自交纯化过程中抗性基因不再分离和丢失，因此不必继续对其自交后代进行抗性选择。研究结果认为 dCAPS标记4451_fon可以作为西瓜抗枯萎病分子辅助选择（molecular marker assisted selection，MAS）的重要工具，能够实现提高选择效率、加速育种进程之目的。
이용국내공개발표적서과항고위병생리소충1기인긴밀련쇄표기4451_fon，수차대아문항고위병육충선출적이배체화사배체충질진행료분자감정，결과표명，전부이배체단계화사배체단계균표현위항병기인형，판정위항병재료，여인공접충감정결과완전일치；분자감정환표명，저사항병단계위항병순합체，재이후적자교순화과정중항성기인불재분리화주실，인차불필계속대기자교후대진행항성선택。연구결과인위 dCAPS표기4451_fon가이작위서과항고위병분자보조선택（molecular marker assisted selection，MAS）적중요공구，능구실현제고선택효솔、가속육충진정지목적。
Molecular identification on diploid and tetraploid watermelon breeding lines which were resistant to Fusarium wilt was carried out with the published dCAPS marker "4451_fon" which was closely linked with resistance gene of Fusarium wilt race 1. The results showed that al the diploid and tetraploid lines expressed as re-sistant genotype, which were defined as Fusarium wilt-resistant materials. The re-sults were consistent with that of artificial inoculation identification. Molecular identifi-cation results also indicated that the resistant lines were homozygote, and the Fusarium wilt-resistant gene would not separate or lose during the future self-crossed purification. Therefore, resistance selection would not be necessary in their progeny populations. The study results thought that dCAPS marker "4451_fon" could be applied on molecular marker assisted selection for Fusarium wilt resistance breeding in watermelon to increase breeding selection efficiency and accelerate breeding progress.