CAJ | 학술논문

作为测定微生物群落功能多样性主要方法之一的 biolog法，由于操作简单、灵敏度高、分辨力强、获得数据量丰富，受到众多研究者的青睐，但文献中报道的有关实验预处理方法却不尽相同。为了筛选最佳的预处理方法，通过比较文献中3种较为常用的方法，研究不同预处理对 biolog法测定微生物群落功能多样性的影响。结果表明：AWCD值总体变化趋势B方法优于 A、C；微生物对六类碳源的利用率中 B方法对大部分碳源利用较高，且结果较稳定；两种处理的 Simpson指数、Shannon丰富度指数及碳源利用丰富度指数均B>C>A，而 McIntosh指数、Shannon均匀度结果不太稳定，但经方差分析，其差异不显著，且 B方法的方差最小；主成分分析的结果 B方法优于 A与 C。总之，在250 r/min的转速下震荡30 min，并于28℃条件下培养接种平板的处理(B法）效果较好，其测定结果重现性好，且操作简便快捷，是比较理想的预处理方法。
작위측정미생물군락공능다양성주요방법지일적 biolog법，유우조작간단、령민도고、분변력강、획득수거량봉부，수도음다연구자적청래，단문헌중보도적유관실험예처리방법각불진상동。위료사선최가적예처리방법，통과비교문헌중3충교위상용적방법，연구불동예처리대 biolog법측정미생물군락공능다양성적영향。결과표명：AWCD치총체변화추세B방법우우 A、C；미생물대륙류탄원적이용솔중 B방법대대부분탄원이용교고，차결과교은정；량충처리적 Simpson지수、Shannon봉부도지수급탄원이용봉부도지수균B>C>A，이 McIntosh지수、Shannon균균도결과불태은정，단경방차분석，기차이불현저，차 B방법적방차최소；주성분분석적결과 B방법우우 A여 C。총지，재250 r/min적전속하진탕30 min，병우28℃조건하배양접충평판적처리(B법）효과교호，기측정결과중현성호，차조작간편쾌첩，시비교이상적예처리방법。
As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper-ation, high sensitivity, strong resolution and rich data. But the preprocessing meth-ods reported in the literatures were not the same. In order to screen the best pre-processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B’s overal trend of AWCD values was better than A and C’s. Method B’s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car-bon source utilization richness index of the two treatments were B>C>A, while the McIntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smal est variance. Method B’s principal component analysis was better than A and C’s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃was the best one, because it was simple, convenient, and with good repeatability.