农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
Agricultural Science & Technology
2015年
10期
2225-2230
,共6页
姚姝%刘燕清%张亚东%朱镇%陈涛%赵庆勇%周丽慧%赵春芳%于新%王才林
姚姝%劉燕清%張亞東%硃鎮%陳濤%趙慶勇%週麗慧%趙春芳%于新%王纔林
요주%류연청%장아동%주진%진도%조경용%주려혜%조춘방%우신%왕재림
稻瘟病%分子标记%抗性基因%多重PCR 体系
稻瘟病%分子標記%抗性基因%多重PCR 體繫
도온병%분자표기%항성기인%다중PCR 체계
Rice blast%Molecular marker%Resistance gene%Multiplex PCR system
稻瘟病是我国水稻主产区的重要病害之一，其主效抗性基因 Pi-ta和 Pi-b在我国很多稻区表现广谱持久的稻瘟病抗性，被广泛应用于我国的水稻育种和生产。本研究选用稻瘟病抗性基因 Pi-ta和 Pi-b及其等位基因的功能标记，在对22份分别已知抗病基因 Pi-ta和Pi-b以及感病基因 pi-ta与 pi-b组成的水稻品种检测验证基础上，建立了2套稻瘟病基因多重 PCR 体系：体系 I同时检测抗病基因Pi-ta与 Pi-b，体系 II同时检测感病基因 pi-ta与pi-b，并利用2套体系对336份高世代育种材料进行检测，与单标记检测结果比较，表现稳定可靠，重复性好。本研究构建的抗稻瘟病基因分子标记多重 PCR体系可用于水稻种质资源的快速评价和抗稻瘟病分子标记辅助育种。
稻瘟病是我國水稻主產區的重要病害之一，其主效抗性基因 Pi-ta和 Pi-b在我國很多稻區錶現廣譜持久的稻瘟病抗性，被廣汎應用于我國的水稻育種和生產。本研究選用稻瘟病抗性基因 Pi-ta和 Pi-b及其等位基因的功能標記，在對22份分彆已知抗病基因 Pi-ta和Pi-b以及感病基因 pi-ta與 pi-b組成的水稻品種檢測驗證基礎上，建立瞭2套稻瘟病基因多重 PCR 體繫：體繫 I同時檢測抗病基因Pi-ta與 Pi-b，體繫 II同時檢測感病基因 pi-ta與pi-b，併利用2套體繫對336份高世代育種材料進行檢測，與單標記檢測結果比較，錶現穩定可靠，重複性好。本研究構建的抗稻瘟病基因分子標記多重 PCR體繫可用于水稻種質資源的快速評價和抗稻瘟病分子標記輔助育種。
도온병시아국수도주산구적중요병해지일，기주효항성기인 Pi-ta화 Pi-b재아국흔다도구표현엄보지구적도온병항성，피엄범응용우아국적수도육충화생산。본연구선용도온병항성기인 Pi-ta화 Pi-b급기등위기인적공능표기，재대22빈분별이지항병기인 Pi-ta화Pi-b이급감병기인 pi-ta여 pi-b조성적수도품충검측험증기출상，건립료2투도온병기인다중 PCR 체계：체계 I동시검측항병기인Pi-ta여 Pi-b，체계 II동시검측감병기인 pi-ta여pi-b，병이용2투체계대336빈고세대육충재료진행검측，여단표기검측결과비교，표현은정가고，중복성호。본연구구건적항도온병기인분자표기다중 PCR체계가용우수도충질자원적쾌속평개화항도온병분자표기보조육충。
Rice blast is one of the important diseases in major rice producing areas of China. The main blast resistance genes Pi-ta and Pi-b showed broad-spectrum and durable resistance to rice blast in many rice growing areas of China, which have been widely utilized in rice breeding and commercial production. In this study, on the basis of detection and verification of the genotypes of 22 rice varieties har-boring known blast resistance genes (Pi-ta and Pi-b) and blast susceptibility genes (pi-ta and pi-b), two multiple PCR systems for these genes were established by us-ing the functional markers of blast resistance genes Pi-ta and Pi-b as wel as blast susceptibility genes pi-ta and pi-b, respectively. Specifical y, multiple PCR system I could simultaneously detect blast resistance genes Pi-ta and Pi-b, while system II could detect simultaneously blast susceptibility genes pi-ta and pi-b. In addition, the genotypes of 336 high generation breeding materials were detected with these two multiple PCR systems. The results were highly consistent with those of conventional single mark detection, indicating that these two multiplex PCR systems were stable, reliable and time-saving. The established multiplex PCR systems may serve as a rapid and efficient method to identify and screen rice germplasm resources and can be applied in marker-assisted selection to polymerize multiple genes for blast resis-tance in rice breeding.
