CAJ | 학술논문

[目的]了解高羊茅 SRP基因结构与功能以及在多种逆境胁迫下的表达变化。[方法]以高羊茅转录组学测序获得的 SRP序列为基础，从高羊茅叶片 cDNA中应用3’ RACE和5’RACE方法扩增出 SRP基因全长 cDNA序列。[结果] SRP基因 cDNA全长为1165 bp，具有一个完整的长度为855 bp的开放阅读框，编码蛋白为284个氨基酸，包含一个REF结构域。通过生物信息学的方法预测SRP基因的结构及功能发现， FaSRP与单子叶植物 SRP蛋白具有相对较高的序列同源性。应用荧光定量 PCR技术分析了 SRP基因在低氮、干旱、高热以及高盐逆境胁迫下的表达变化发现 SRP基因能够应答低氮、干旱、高温等胁迫，但应答机制不一样，可能是通过不同途径调节植物抗逆性，FaSRP对高盐胁迫不敏感。[结论]该研究为培育抗旱、耐热、营养高效的高羊茅品种提供候选基因与技术储备。
[목적]료해고양모 SRP기인결구여공능이급재다충역경협박하적표체변화。[방법]이고양모전록조학측서획득적 SRP서렬위기출，종고양모협편 cDNA중응용3’ RACE화5’RACE방법확증출 SRP기인전장 cDNA서렬。[결과] SRP기인 cDNA전장위1165 bp，구유일개완정적장도위855 bp적개방열독광，편마단백위284개안기산，포함일개REF결구역。통과생물신식학적방법예측SRP기인적결구급공능발현， FaSRP여단자협식물 SRP단백구유상대교고적서렬동원성。응용형광정량 PCR기술분석료 SRP기인재저담、간한、고열이급고염역경협박하적표체변화발현 SRP기인능구응답저담、간한、고온등협박，단응답궤제불일양，가능시통과불동도경조절식물항역성，FaSRP대고염협박불민감。[결론]해연구위배육항한、내열、영양고효적고양모품충제공후선기인여기술저비。
Objective] This study aimed to study the structure and functions of SRP gene and variation in its expression under abiotic stresses. [Method] Using the SRP sequence obtained from transcriptome sequencing as the template, the ful-length cDNA sequence of SRP gene in Festuca arundinacea was amplified using the 3’RACE and 5’RACE methods. [Result] The cDNA sequence of SRP gene has a ful length of 1 165 bp, and it contains an open reading frame in ful length of 855 bp. The encoded protein by SRP gene is composed of 284 amino acids, and contains a REF domain. The bioinformatic researches on structures and functions of SRPs show that the SRP gene in Festuca arundinacea (FaSRP) has relatively high ho-mologies with SRP s in monocots. Under low nitrogen, drought, high temperature and high salt stresses, the variations in expression of FaSRP gene were studied using fluorescence quantitative PCR. The results showed that FaSRP gene makes responses to low nitrogen, drought and high temperature stresses, but the relevant response mechanisms are not the same, indicating different pathways regulating re-sistance of plants. The expression of FaSRP gene is insensitive to high salt stress. [Conclusion] This study wil provide certain candidate gene and technical reserve for breeding of drought- and high temperature-tolerant, nutritious and highly efficient Festuca arundinacea cultivars.