CAJ | 학술논문

目的：探讨肿瘤坏死因子-α（tumor necrosis factor alpha，TNF-α）的基因多态性及环境因素在新疆哈萨克族食管癌家族聚集中的交互作用。方法：在新疆北部食管癌高发区选取43例新疆哈萨克族食管癌家族成员及40例对照家族成员为研究对象，采用PCR-RFLP方法检测两组研究对象的外周血TNF-α308位点的基因型，对研究对象进行环境因素调查。结果：在新疆哈萨克族食管癌家族和非食管癌家族成员中TNF-α308位点的不同基因型分布差异有统计学意义（P＝0.004，OR＝8.786，95％CI：1.824～12.748），在食管癌家族成员中TNF-α308位点基因携带突变基因AA型和GA型频率明显增高，是非食管癌家族成员的8.786倍；在食管癌患者与非患者之间TNF-α308位点的GG、GA、AA基因型分布差异有统计学意义（P＝0.031，OR＝2.821，95％CI为1.722～11.016），与GG型相比，突变型基因可显著增加新疆哈萨克族食管癌的发病风险。分析TNF-α308位点的不同基因型与环境因素的交互作用，提示TNF-α308位点的基因多态性与饮酒（P＝0.016，OR＝1.531，95％ CI：0.143～1.897）、暴饮暴食（P＝0.021，OR＝1.340，95％ CI：0.266～3.789）在新疆哈萨克族食管癌家族发病可能起到交互作用。结论：TNF-α308位点基因多态性在新疆哈萨克族食管癌家族聚集中起一定作用。TNF-α308位点的基因多态性与饮酒、暴饮暴食在新疆哈萨克族食管癌家族发病中起到共同作用，可能与新疆哈萨克族食管癌高发有关。
목적：탐토종류배사인자-α（tumor necrosis factor alpha，TNF-α）적기인다태성급배경인소재신강합살극족식관암가족취집중적교호작용。방법：재신강북부식관암고발구선취43례신강합살극족식관암가족성원급40례대조가족성원위연구대상，채용PCR-RFLP방법검측량조연구대상적외주혈TNF-α308위점적기인형，대연구대상진행배경인소조사。결과：재신강합살극족식관암가족화비식관암가족성원중TNF-α308위점적불동기인형분포차이유통계학의의（P＝0.004，OR＝8.786，95％CI：1.824～12.748），재식관암가족성원중TNF-α308위점기인휴대돌변기인AA형화GA형빈솔명현증고，시비식관암가족성원적8.786배；재식관암환자여비환자지간TNF-α308위점적GG、GA、AA기인형분포차이유통계학의의（P＝0.031，OR＝2.821，95％CI위1.722～11.016），여GG형상비，돌변형기인가현저증가신강합살극족식관암적발병풍험。분석TNF-α308위점적불동기인형여배경인소적교호작용，제시TNF-α308위점적기인다태성여음주（P＝0.016，OR＝1.531，95％ CI：0.143～1.897）、폭음폭식（P＝0.021，OR＝1.340，95％ CI：0.266～3.789）재신강합살극족식관암가족발병가능기도교호작용。결론：TNF-α308위점기인다태성재신강합살극족식관암가족취집중기일정작용。TNF-α308위점적기인다태성여음주、폭음폭식재신강합살극족식관암가족발병중기도공동작용，가능여신강합살극족식관암고발유관。
Objective:To investigate the interaction of polymorphisms of TNF-αand environmental factors in path-ogenesis of esophageal cancer families in Kazak in Xinjiang Province.Methods:In the northern part of Xinjiang,blood samples of 43 family members from esophageal cancer families in Kazak and 40 members from control families were collect-ed .Genotype of TNF-α308 were determined by PCR-RFLP technology.Environmental factors were inquired by question-naire.Results:There was significant statistic difference between Kazakh esophageal family and control family in the distri-bution frequency of the different gene type of TNF-α308(P=0.004,OR=8.786,95%CI:1.824~12.748).The genotype frequencies of AA and GA of TNF-α308 were 8.786 times higher in the Xinjiang Kazakh esophageal families than those of the control families with significant difference.Genotypes frequencies of GG、GA and AA at TNF-α308 were significantly different(P=0.031,OR=2.821,95%CI:1.722~11.016)between esophageal cancer patients and non-esophageal cancer patients.Mutant gene significantly increased the risk of developing esophageal cancer comparing with GG genotype.Analy-sis of interaction between the polymorphism of TNF-α308 and environmental factors revealed that alcohol,overeating and TNF-α308 gene polymorphism had a multiplicative interac-tion in Kazakh esophageal cancer families.Conclusion:The polymorphism of TNF-α308 gene plays a role in the family aggregation of esophageal cancer in Xinjiang Kazakh.Alco-hol,overeating and the polymorphism genotype of TNF-α308 are likely to play a multiplicative interaction in esophageal families and may be related to the high incidence of esophageal cancer in Xinjiang Kazakh.