实验技术与管理
實驗技術與管理
실험기술여관리
Experimental Technology and Management
2015年
11期
66-68
,共3页
血清淀粉酶%溯源%检测系统%方法分组
血清澱粉酶%溯源%檢測繫統%方法分組
혈청정분매%소원%검측계통%방법분조
serum amylase%traceability%detection system%method group
从一项血清酶活性可溯源性研究中发现淀粉酶(Amylase,AMY)测定结果与校正物的定值相差悬殊,并且不同来源的试剂盒间也存在高低不一的系统误差这一现象出发,分析了出现这些系统误差的原因,从理论和实验两个方面指出影响 AMY 测定结果的关键因素是 AMY 作用底物的结构不同,以此为例进而说明血清酶活性结果的溯源必须考虑构成检测系统的各个要素,其中首先是方法学原理及试剂盒的性能质量。
從一項血清酶活性可溯源性研究中髮現澱粉酶(Amylase,AMY)測定結果與校正物的定值相差懸殊,併且不同來源的試劑盒間也存在高低不一的繫統誤差這一現象齣髮,分析瞭齣現這些繫統誤差的原因,從理論和實驗兩箇方麵指齣影響 AMY 測定結果的關鍵因素是 AMY 作用底物的結構不同,以此為例進而說明血清酶活性結果的溯源必鬚攷慮構成檢測繫統的各箇要素,其中首先是方法學原理及試劑盒的性能質量。
종일항혈청매활성가소원성연구중발현정분매(Amylase,AMY)측정결과여교정물적정치상차현수,병차불동래원적시제합간야존재고저불일적계통오차저일현상출발,분석료출현저사계통오차적원인,종이론화실험량개방면지출영향 AMY 측정결과적관건인소시 AMY 작용저물적결구불동,이차위례진이설명혈청매활성결과적소원필수고필구성검측계통적각개요소,기중수선시방법학원리급시제합적성능질량。
It was found from a traceability study of serum enzyme activity that the Amylase (Amylase,AMY) measurement results differ greatly on the value determination of calibrator,and there are also either more or less systemic errors between kits from different sources.The causes of the systemic error are analyzed.And from two aspects of theory and experiment it was proved that the key factors for affecting AMY measurement results are structural difference of AMY substrate.It is a good example to demonstrate that primary factors of a detection system,first of all, the methodological principles and kit performance and quality must be considered for serum amylase measuring results traceability.
