CAJ | 학술논문

采用变性梯度凝胶电泳技术（denatured gradient gel electrophoresis，DGGE）对不同盐渍白萝卜样品的真菌菌群结构分析表明，犆犪狀犱犻犱犪犾犪犮狋犻狊-犮狅狀犱犲狀狊犻、犆犪狀犱犻犱犪狏犲狉狊犪狋犻犾犻狊、膜醭毕赤酵母（犘犻犮犺犻犪犿犲犿犫狉犪狀犻犳犪犮犻犲狀狊）、汉逊德巴氏酵母菌（犇犲犫犪狉狔狅犿狔犮犲狊犺犪狀狊犲狀犻犻）为盐渍萝卜的优势菌。通过传统分离培养技术从样品中分离到17株酵母菌，通过分子生物学鉴定为犘犻犮犺犻犪sp 。、膜醭毕赤酵母（犘犻犮犺犻犪犿犲犿犫狉犪狀犲犳犪犮犻犲狀狊）、德巴利酵母属（犇犲犫犪狉狔狅犿狔犮犲狊sp 。）。传统分离培养技术未分离到犆犪狀犱犻犱犪犾犪犮狋犻狊-犮狅狀犱犲狀狊犻，犆犪狀犱犻犱犪狏犲狉狊犪狋犻犾犻狊。DGGE技术能够快速高效、直观地反映盐渍白萝卜的菌群结构，结果合理。
채용변성제도응효전영기술（denatured gradient gel electrophoresis，DGGE）대불동염지백라복양품적진균균군결구분석표명，직규장환패환규은규발의패격-발경장환시장격패、직규장환패환규이시비격규의패은패격、막복필적효모（마패발강패규변시변주비규장패표규발패시장격）、한손덕파씨효모균（분시주규비니경변니발시격강규장격시장패패）위염지라복적우세균。통과전통분리배양기술종양품중분리도17주효모균，통과분자생물학감정위마패발강패규sp 。、막복필적효모（마패발강패규변시변주비규장시표규발패시장격）、덕파리효모속（분시주규비니경변니발시격sp 。）。전통분리배양기술미분리도직규장환패환규은규발의패격-발경장환시장격패，직규장환패환규이시비격규의패은패격。DGGE기술능구쾌속고효、직관지반영염지백라복적균군결구，결과합리。
The fungi communities of different salted Raphanus sativus are analyzed by denatured gradient gel electrophoresis (DGGE ).The dominant fungi groups of salted Raphanus sativus are Candida lactis-condensi,Candida versatilis,Candida sp.,Pichi sp.,the closest species are Candida lactis-condensi,Candida versatilis,Pichia membranifaciens,Debaryomyces hansenii.17 yeasts are isolated from salted Raphanus sativus by cultivation methods,and are identified by molecular biology methods,the strains are Pichi sp.,Pichia membranefaciens,and Debaryomyces hansenii.Candida lactis-condensi and Candida versatilis are not isolated with the cultivation methods.DGGE is a suitable tool for the analysis of fungi communities of salted Raphanus sativus,as it is more rapid and comprehensive than cultivation methods detection of species.