CAJ | 학술논문

采用对峙培养法从来自土壤的136株细菌中筛选得到对火龙果溃疡病菌有拮抗作用的菌株HTN-5，抑菌带宽9.0 mm。通过形态学、生理生化特性和16s rDNA序列分析，将拮抗菌HTN-5初步鉴定为皮氏类芽孢杆菌Paenibacillus peoriae。从8种培养基中筛选出IFFI培养基最有利于菌株HTN-5形成抑菌活性物质，正交试验优化其成分，得最优配方为：牛肉膏2.50 g/L，蛋白胨10.00 g/L，葡萄糖12.50 g/L，乳糖10.00 g/L，酵母膏2.50 g/L，氯化钠5.00g/L， pH 6.8。
채용대치배양법종래자토양적136주세균중사선득도대화룡과궤양병균유길항작용적균주HTN-5，억균대관9.0 mm。통과형태학、생리생화특성화16s rDNA서렬분석，장길항균HTN-5초보감정위피씨류아포간균Paenibacillus peoriae。종8충배양기중사선출IFFI배양기최유리우균주HTN-5형성억균활성물질，정교시험우화기성분，득최우배방위：우육고2.50 g/L，단백동10.00 g/L，포도당12.50 g/L，유당10.00 g/L，효모고2.50 g/L，록화납5.00g/L， pH 6.8。
The isolate HTN-5, which antagonized against Neoscytalidium dimidiatum causing pitaya canker, was screened from one hundred and thirty-six bacteria which isolated from the diseased orchard soil. The width of inhibiting zone between HTN-5 and Neoscytalidium dimidiatum reached 9.0 mm. HTN-5 was identified as Paenibacillus peoriae based on morphology, physio-biochemical reactions and 16s rDNA sequences. The IFFI medium was the most suitable medium for isolate HTN-5 to produce antimicrobial active substances, and the optimal medium ingredients, was obtained by the L16 (45) Latin orthogonal experiments, which were comprised of beef extract 2.50 g/L, peptone 10.00 g/L glucose 12.50 g/L, lactose 10.00 g/L, yeast extract 2.50 g/L, sodium chloride 5.00 g/L, pH6.8.