CAJ | 학술논문

丝素蛋白具有良好的生物相容性及降解性，被广泛地用于生物材料。本研究以废蚕茧为原料，通过脱胶、溶解、透析、浓缩等步骤制备了丝素蛋白溶液，再用木瓜蛋白酶降解丝素蛋白，利用SDS－聚丙烯酰胺凝胶电泳（SDS－PAGE）进行丝素蛋白多肽的鉴定，探讨pH、温度及酶活等因素对木瓜蛋白酶降解丝素蛋白影响。结果表明：在pH8～9、温度60～70℃，木瓜蛋白酶的活性为20～25 U时丝素蛋白水解效果较好，酶的作用时间越长效果越好。本研究为开发丝素蛋白在食品、化妆品中的提供了基础。
사소단백구유량호적생물상용성급강해성，피엄범지용우생물재료。본연구이폐잠충위원료，통과탈효、용해、투석、농축등보취제비료사소단백용액，재용목과단백매강해사소단백，이용SDS－취병희선알응효전영（SDS－PAGE）진행사소단백다태적감정，탐토pH、온도급매활등인소대목과단백매강해사소단백영향。결과표명：재pH8～9、온도60～70℃，목과단백매적활성위20～25 U시사소단백수해효과교호，매적작용시간월장효과월호。본연구위개발사소단백재식품、화장품중적제공료기출。
Silk fibroin is widely used in biomaterials based on its good biocompatibility and degradability .The silk fibroin solution is prepared by the steps of the process of degluing ,dissolution ,dialysis and concentration from waste cocoons . Then the fibroin is degraded by papain .The best condition of the degradation is explored by the means of SDS -PAGE for identifying the factors of pH ,temperature and papain activity .The results show that under the circumstances of pH being 8~9 ,temperature 60-70℃ ,and papain activity 20~25 U ,the hydrolysis effect of silk fibroin was the best ,and the longer time ,the better effect .