CAJ | 학술논문

近年来，重金属污染引起了人们广泛关注。传统的重金属检测方法需要依赖大型仪器，预处理繁琐耗时，因此急需发展重金属的快速高灵敏检测技术。基于金纳米颗粒（AuNPs）的比色法传感器具有分析操作简单、灵敏度高、成本低等特点，在环境监测、食品安全以及化学和生物分析领域得到了广泛关注和应用。本文基于纳米金（AuNPs）特性与Fenton反应原理，构建了一种用于水中Cu2＋检测的简单快速、高灵敏检测方法。该方法利用Cu2＋与抗坏血酸钠（sodium ascorbate ，SA）发生Fenton反应，生成具有强氧化性的羟基自由基（?OH），?OH将附着在纳米金表面的单链DNA（ssDNA）裂解，使得金纳米粒子失去保护，容易在一定的盐浓度下发生聚集，从而引起吸光度值的变化。实验结果表明，最优的反应条件为p H 7.9，11 mg?L －1 ssDNA ，8 mmol?L －1 SA以及70 mmol?L －1 NaCl ，金纳米粒子在700和525 nm处的吸光度比值（A700／A525）与Cu2＋浓度成线性关系。该方法对Cu2＋检测线性范围为0.1～10.0μmol ?L －1，检出限为24 nmol?L －1（3σ）。对饮用水，自来水及湖水的Cu2＋加标回收率可以达到87％～120％，表明该方法能够用于实际水样的C u2＋检测。
근년래，중금속오염인기료인문엄범관주。전통적중금속검측방법수요의뢰대형의기，예처리번쇄모시，인차급수발전중금속적쾌속고령민검측기술。기우금납미과립（AuNPs）적비색법전감기구유분석조작간단、령민도고、성본저등특점，재배경감측、식품안전이급화학화생물분석영역득도료엄범관주화응용。본문기우납미금（AuNPs）특성여Fenton반응원리，구건료일충용우수중Cu2＋검측적간단쾌속、고령민검측방법。해방법이용Cu2＋여항배혈산납（sodium ascorbate ，SA）발생Fenton반응，생성구유강양화성적간기자유기（?OH），?OH장부착재납미금표면적단련DNA（ssDNA）렬해，사득금납미입자실거보호，용역재일정적염농도하발생취집，종이인기흡광도치적변화。실험결과표명，최우적반응조건위p H 7.9，11 mg?L －1 ssDNA ，8 mmol?L －1 SA이급70 mmol?L －1 NaCl ，금납미입자재700화525 nm처적흡광도비치（A700／A525）여Cu2＋농도성선성관계。해방법대Cu2＋검측선성범위위0.1～10.0μmol ?L －1，검출한위24 nmol?L －1（3σ）。대음용수，자래수급호수적Cu2＋가표회수솔가이체도87％～120％，표명해방법능구용우실제수양적C u2＋검측。
Heavy metal pollution has received great attentions in recent years .The traditional methods for heavy metal detection rely on the expensive laboratory instruments and need time‐consuming preparation steps ;therefore ,it is urgent to develop quick and highly sensitive new technologies for heavy metal detection . The colorimetric method based on the gold nanoparticles (AuNPs) features with simple operation ,high sensitivity and low cost ,therefore ,enabling it widely concerned and used in the environmental monitoring ,food safety and chemical and biological sensing fields .This work developed a simple ,rapid and highly sensitive strategy based on the Fenton reaction and unmodified AuNPs for the detection of Cu2+ in water samples .The hydroxyl radical (?OH) generated by the Fenton reaction between the Cu2+ and sodium ascorbate (SA) oxidized the single stranded DNA (ssDNA) attached on the AuNPs surface into variable sequence fragments .The cleavage of ssDNA induced the aggregation of AuNPs in a certain salt solution ,therefore ,resulting in the changes on the absorbance of solution .The assay conditions were optimized to be pH value of 7.9 ,11 mg?L -1 ssDNA ,8 mmol?L -1 SA and 70 mmol?L -1 NaCl .Results showed that the ab‐sorbance ratio values at the wavelengths of 700 and 525 nm (A700/A525 ) were linearly correlated with the Cu2+ concentrations . The linear detection range was 0.1~10.0μmol?L -1 with a detection limit of 24 nmol?L -1 (3σ) .Spiked recoveries ranged from 87% ~120% in three sorts of water ,including drinking water ,tap water and lake water ,which confirmed that the potentials of the proposed assay for Cu2+ detection in reality .