CAJ | 학술논문

本研究对益生菌菌株副干酪乳杆菌L9（Lactobacillus paracasei L9）进行了体外及体内的安全性评价。利用体外试验评价了L9的抗生素抗敏感性及生物胺的生成能力；体内试验评价了L9的经口毒性、正常状况下的易位风险及腹腔注射模拟的极端情况下的易位风险。结果表明，菌株L9不存在种属特有耐性以外的抗生素耐受性，不存在抗生素转移的风险；在脱羧酶培养基中培养未发现生物胺的产生；以108、109、1010 cfu/鼠灌胃28d，小鼠未发生不良反应，未发生向血液、肝脏、脾脏和肾脏的易位；109cfu/鼠腹腔注射发生易位现象，但未发生菌血症并且易位不会造成疾病或导致死亡，同时易位状况随时间有所缓解，表现为较高的易位安全性。试验结果证实副干酪乳杆菌L9具有较高安全性，可以直接应用于工业化生产。
본연구대익생균균주부간락유간균L9（Lactobacillus paracasei L9）진행료체외급체내적안전성평개。이용체외시험평개료L9적항생소항민감성급생물알적생성능력；체내시험평개료L9적경구독성、정상상황하적역위풍험급복강주사모의적겁단정황하적역위풍험。결과표명，균주L9불존재충속특유내성이외적항생소내수성，불존재항생소전이적풍험；재탈최매배양기중배양미발현생물알적산생；이108、109、1010 cfu/서관위28d，소서미발생불량반응，미발생향혈액、간장、비장화신장적역위；109cfu/서복강주사발생역위현상，단미발생균혈증병차역위불회조성질병혹도치사망，동시역위상황수시간유소완해，표현위교고적역위안전성。시험결과증실부간락유간균L9구유교고안전성，가이직접응용우공업화생산。
The safety ofLactobacillus paracasei L9 was evaluated. In our invitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. That this strain did not possess transferable antibiotics resistance, and it did not produce biogenic amine. The oral toxicity of this strain was evaluated in Balb/C mice. Oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. In trapertoneal administration caused a signiifcant translocation to liver, spleen and kidney. However, this translocation did not cause illness or death throughout the experiment. The results showed that L.paracaseiL9 is likely to be safe for human consumption.