CAJ | 학술논문

目的：探讨HMGB1在肺泡上皮细胞间质转分化中的作用。方法设计并合成针对HMGB1的小干扰RNA （siRNA），体外培养Ⅱ型肺泡上皮细胞系细胞-A549细胞，将细胞分为4组：1）对照组，2）TGF-β1刺激组，3）RNAi组（TGF-β1＋HMGB1 siRNA），4）RNAi阴性对照组（TGF-β1＋siRNA阴性对照）；倒置相差显微镜观察细胞形态学；RT-PCR法检测HMGB1和α平滑肌肌动蛋白（α-SMA）的mRNA表达；Western blot法检测α-SMA和HMGB1蛋白表达。结果 TGF-β1刺激组细胞HMGB1和α-SMA的mRNA和蛋白表达均较对照组显著增加（ P＜0.01）；RNAi组HMGB1、α-SMA mRNA和蛋白表达均较正常对照组显著下降（ P＜0.01）。结论 HMGB1可能参与了TGFβ1诱导的肺泡上皮细胞转分化。
목적：탐토HMGB1재폐포상피세포간질전분화중적작용。방법설계병합성침대HMGB1적소간우RNA （siRNA），체외배양Ⅱ형폐포상피세포계세포-A549세포，장세포분위4조：1）대조조，2）TGF-β1자격조，3）RNAi조（TGF-β1＋HMGB1 siRNA），4）RNAi음성대조조（TGF-β1＋siRNA음성대조）；도치상차현미경관찰세포형태학；RT-PCR법검측HMGB1화α평활기기동단백（α-SMA）적mRNA표체；Western blot법검측α-SMA화HMGB1단백표체。결과 TGF-β1자격조세포HMGB1화α-SMA적mRNA화단백표체균교대조조현저증가（ P＜0.01）；RNAi조HMGB1、α-SMA mRNA화단백표체균교정상대조조현저하강（ P＜0.01）。결론 HMGB1가능삼여료TGFβ1유도적폐포상피세포전분화。
Objective To investigate the role of HMGB 1 in epithelial-mesenchymal transition .Methods Specific siRNA of HMGB1 was designed and synthesized .Cultured typeⅡalveolar epithelial cell line-A549 cells were divided into 4 groups:1)control group, 2)model group induced by TGF-β1, 3)HMGB1 RNAi group, 4)RNAi negative con-trol group .Cellular morphology changes were observed by phase-contrast microscope .HMGB1 andα-SMA expression in A549 cells was detected by RT-PCR and Western blotting respectively .Results mRNA and protein expression of HMGB1 andα-SMA in TGF-β1 group increased significantly than that in control group (P<0.01).HMGB1,α-SMA mRNA and protein expression in siRNA-treated cells decreased significantly as compared with that in TGF -β1 group (P<0.01).Conclusions HMGB1 may be involved in the TGF-β1 induced EMT.