CAJ | 학술논문

将转正、反义无机焦磷酸酶（PPase）基因马铃薯‘甘农薯2号’块茎分别贮藏于4℃和25℃下90 d，测定其PPase活性和无机Pi含量等生理生化指标。结果表明，在25℃贮藏温度下，与未转基因对照相比，转正义PPase基因的两个株系（G-2-4和G-2-5）块茎中PPase活性、Pi与可溶性糖含量均增加，与未转基因对照相比差异显著， G-2-4的淀粉含量与未转基因对照相比显著降低。转反义PPase基因的两个株系（G-1-1和G-1-2）PPase活性、Pi与可溶性糖含量均降低，与未转基因对照相比差异显著，G-1-2淀粉含量与未转基因对照相比极显著增加。与25℃贮藏温度相比，4℃贮藏温度下转正、反义基因株系块茎中的PPase活性、Pi、可溶性糖和淀粉含量的变化趋势与25℃贮藏温度基本一致，但其PPase活性、Pi含量和淀粉含量高于25℃贮藏温度下的转基因株系块茎，而其可溶性糖含量低于25℃贮藏温度下的转基因株系块茎含量。
장전정、반의무궤초린산매（PPase）기인마령서‘감농서2호’괴경분별저장우4℃화25℃하90 d，측정기PPase활성화무궤Pi함량등생리생화지표。결과표명，재25℃저장온도하，여미전기인대조상비，전정의PPase기인적량개주계（G-2-4화G-2-5）괴경중PPase활성、Pi여가용성당함량균증가，여미전기인대조상비차이현저， G-2-4적정분함량여미전기인대조상비현저강저。전반의PPase기인적량개주계（G-1-1화G-1-2）PPase활성、Pi여가용성당함량균강저，여미전기인대조상비차이현저，G-1-2정분함량여미전기인대조상비겁현저증가。여25℃저장온도상비，4℃저장온도하전정、반의기인주계괴경중적PPase활성、Pi、가용성당화정분함량적변화추세여25℃저장온도기본일치，단기PPase활성、Pi함량화정분함량고우25℃저장온도하적전기인주계괴경，이기가용성당함량저우25℃저장온도하적전기인주계괴경함량。
Tubers of the transgenic sense and antisense PPase gene of the potato cultivar 'Gannongshu 2' were stored at 4℃and 25℃for 90 days, respectively. PPase activity, Pi content and other physiological indexes related to tuber dormancy were measured. The results showed that when tubers were stored at 25℃, PPase activity, Pi and soluble sugar contents of two transgenic sense lines (G-2-4 and G-2-5) were higher than those of the non-transgenic plants, and the difference was significant. The starch content of G-2-4 was obviously lower compared with non-transgenic plants, and the difference was significant. Two transgenic antisense lines (G-1-1 and G-1-2) exhibited obviously lower PPase activity, Pi and soluble sugar contents compared with non-transgenic plants, and the differences were highly significant. The starch content of G-1-2 was highly significantly higher than that of non-transgenic plants. The changing trend of the physiological indexes of the transgenic sense and antisense potato tubers under 4℃was similar to those stored under 25℃. However, the transgenic potato tubers exhibited higher PPase activity, Pi and starch contents, while soluble sugar content was lower than those stored under 25℃.