CAJ | 학술논문

目的：观察和比较广藿香油及其主要成分广藿香醇、广藿香酮对斑马鱼胚胎发育的毒性。方法：分别将发育10h 的胚胎置于梯度浓度的广藿香油、广藿香醇和广藿香酮中培养，于24h、48h、72h、96h 观察和统计各组斑马鱼胚胎的死亡率、畸形率、胚胎形态、摆尾次数和心率。结果：随作用时间延长，三种药物毒性表现越明显；24h 时广藿香醇和广藿香酮对斑马鱼的 LD50分别为50.3、12.9mg／L，广藿香油组无明显致死现象；96h 时广藿香油、广藿香醇和广藿香酮对斑马鱼胚胎的 LC50分别为21.22、12.863、11.76mg／L。对比12mg／L 浓度下三种药物的毒性，广藿香醇和广藿香酮在24h 时主要表现为生长缓慢、止于体节期，96h 时表现为导致幼鱼脊柱侧弯，心脏肿大，卵黄囊肿大、摆尾次数和心率明显下降等现象；广藿香油在各时间点主要表现为延缓黑色素的生成和轻微的心脏毒性；对比结果表明，广藿香酮毒性最明显。结论：广藿香油、广藿香醇和广藿香酮对斑马鱼均有胚胎发育毒性，毒性大小：广藿香酮＞广藿香醇＞广藿香油。
목적：관찰화비교엄곽향유급기주요성분엄곽향순、엄곽향동대반마어배태발육적독성。방법：분별장발육10h 적배태치우제도농도적엄곽향유、엄곽향순화엄곽향동중배양，우24h、48h、72h、96h 관찰화통계각조반마어배태적사망솔、기형솔、배태형태、파미차수화심솔。결과：수작용시간연장，삼충약물독성표현월명현；24h 시엄곽향순화엄곽향동대반마어적 LD50분별위50.3、12.9mg／L，엄곽향유조무명현치사현상；96h 시엄곽향유、엄곽향순화엄곽향동대반마어배태적 LC50분별위21.22、12.863、11.76mg／L。대비12mg／L 농도하삼충약물적독성，엄곽향순화엄곽향동재24h 시주요표현위생장완만、지우체절기，96h 시표현위도치유어척주측만，심장종대，란황낭종대、파미차수화심솔명현하강등현상；엄곽향유재각시간점주요표현위연완흑색소적생성화경미적심장독성；대비결과표명，엄곽향동독성최명현。결론：엄곽향유、엄곽향순화엄곽향동대반마어균유배태발육독성，독성대소：엄곽향동＞엄곽향순＞엄곽향유。
Objective To study the comparation of oil of Pogostemon cablin and its main monomer in Development of Zebrafish Embryos from 0 to 96h post fertilization (96h).Methods The embryos exposure ladder concentrations for the oil of Pogostemon cab-lin,Patchouli and Pogostone at 10h.It were cultured for 96h.LC50,relative survival rate,spontaneous movement of embryos heart beat rate and morphologic change were detected in 10h zebrafish embryos with incubation of the oil of Pogostemon cablin and its main monomer for 96h.Results Control group was established.With the extended time,the toxicity was more obviousely.24h,the LC50 value of the oil ofPogostemon cablin in the zebrafish embryos couldn't calculate during the exposure concentrations.The LC50 values of Patchouli and Pogostone were 50.3、12.9mg/L,respectively;24h,The LC50 values of the oil ofPogostemon cablin,Patchouli and Pogostone were 21.2、12.9、11.8mg/L,respectively.12 mg/L for the oil ofPogostemon cablin,Patchouli and Pogostone caused malformations in development of embryos,24h,the embryos was hypoplasia and during the somites;96h,zebrafish larvae with the characteristic of notochord malformation,cardiac edema,yolk edema.The three drugs inhibited time -dependent,especially Pogos-tone;24h,Patchouli and Pogostone caused the reduction of spontaneous movement and heart rate,the oil ofPogostemon cablin had no effect on it.Conclusion The oil of Pogostemon cablin,Patchouli and Pogostone had toxicity on the development of zebrafish embryos, and the toxicity of zebrafish embryos :the oil of Pogostemon cablin >Patchouli >Pogostone.