湖南中医药大学学报
湖南中醫藥大學學報
호남중의약대학학보
Journal of Traditional Chinese Medicine University of Hunan
2015年
11期
13-17
,共5页
李点%王超群%廖亮英%胡平%彭银艳
李點%王超群%廖亮英%鬍平%彭銀豔
리점%왕초군%료량영%호평%팽은염
干眼%养阴润目丸%结膜上皮细胞%ICAM-1%p38MAPK%p-p38MAPK
榦眼%養陰潤目汍%結膜上皮細胞%ICAM-1%p38MAPK%p-p38MAPK
간안%양음윤목환%결막상피세포%ICAM-1%p38MAPK%p-p38MAPK
dry eye%Yangyin Runmu Pills%conjunctival epithelial cell%ICAM-1%p38MAPK%p-p38MAPK
目的:研究养阴润目丸对干眼模型大鼠结膜上皮细胞中细胞间粘附分子-1(ICAM-1)、丝裂原活化蛋白激酶p38(p38MAPK)、磷酸化-丝裂原活化蛋白激酶p38(p-p38MAPK)表达的影响。方法将60只SD雄性大鼠随机分为5组(正常组、假手术组、养阴润目丸组、新泪然组、模型组),每组12只。除正常组与假手术组外均采用去势法制造干眼模型。造模成功后一周开始分别给药治疗。用药3个月后,分别进行泪液分泌试验(SIT)及泪膜破裂时间(BUT)的检测,取大鼠结膜上皮细胞,采用免疫组化法检测p38MAPK的表达,采用Western blot法检测ICAM-1、p-p38MAPK的表达。结果与模型组比较,养阴润目丸组SIT明显增多,BUT明显延长;养阴润目丸可显著减少结膜上皮细胞中ICAM-1、p38MAPK、p-p38MAPK的表达,差异有统计学意义(P<0.01)。结论养阴润目丸可有效改善眼表症状,增加泪液分泌量,延长泪膜破裂时间,下调结膜上皮细胞中ICAM-1、p38MAPK、p-p38MAPK的表达。提示干眼的发病机制与炎症及p38MAPK信号通路相关,养阴润目丸对干眼的治疗具有一定的应用前景。
目的:研究養陰潤目汍對榦眼模型大鼠結膜上皮細胞中細胞間粘附分子-1(ICAM-1)、絲裂原活化蛋白激酶p38(p38MAPK)、燐痠化-絲裂原活化蛋白激酶p38(p-p38MAPK)錶達的影響。方法將60隻SD雄性大鼠隨機分為5組(正常組、假手術組、養陰潤目汍組、新淚然組、模型組),每組12隻。除正常組與假手術組外均採用去勢法製造榦眼模型。造模成功後一週開始分彆給藥治療。用藥3箇月後,分彆進行淚液分泌試驗(SIT)及淚膜破裂時間(BUT)的檢測,取大鼠結膜上皮細胞,採用免疫組化法檢測p38MAPK的錶達,採用Western blot法檢測ICAM-1、p-p38MAPK的錶達。結果與模型組比較,養陰潤目汍組SIT明顯增多,BUT明顯延長;養陰潤目汍可顯著減少結膜上皮細胞中ICAM-1、p38MAPK、p-p38MAPK的錶達,差異有統計學意義(P<0.01)。結論養陰潤目汍可有效改善眼錶癥狀,增加淚液分泌量,延長淚膜破裂時間,下調結膜上皮細胞中ICAM-1、p38MAPK、p-p38MAPK的錶達。提示榦眼的髮病機製與炎癥及p38MAPK信號通路相關,養陰潤目汍對榦眼的治療具有一定的應用前景。
목적:연구양음윤목환대간안모형대서결막상피세포중세포간점부분자-1(ICAM-1)、사렬원활화단백격매p38(p38MAPK)、린산화-사렬원활화단백격매p38(p-p38MAPK)표체적영향。방법장60지SD웅성대서수궤분위5조(정상조、가수술조、양음윤목환조、신루연조、모형조),매조12지。제정상조여가수술조외균채용거세법제조간안모형。조모성공후일주개시분별급약치료。용약3개월후,분별진행루액분비시험(SIT)급루막파렬시간(BUT)적검측,취대서결막상피세포,채용면역조화법검측p38MAPK적표체,채용Western blot법검측ICAM-1、p-p38MAPK적표체。결과여모형조비교,양음윤목환조SIT명현증다,BUT명현연장;양음윤목환가현저감소결막상피세포중ICAM-1、p38MAPK、p-p38MAPK적표체,차이유통계학의의(P<0.01)。결론양음윤목환가유효개선안표증상,증가루액분비량,연장루막파렬시간,하조결막상피세포중ICAM-1、p38MAPK、p-p38MAPK적표체。제시간안적발병궤제여염증급p38MAPK신호통로상관,양음윤목환대간안적치료구유일정적응용전경。
〔Abstract〕 Objective To study the effect of Yangyin Runmu Pills on the expression of intercellular adhesion molecule-1 (ICAM-1), p38 mitogen-activated Protein kinase (p38MAPK), phospho-and mitogen activated protein kinase p38 antibody (p-p38MAPK) in conjunctival epithelial cells of dry eye model rats. Methods Sixty Sprague-Dawley (SD) male rats were randomly divided into five groups (the normal group, sham operation group, Yangyin Runmu Pills group, Xinleiran group, model group), and 12 rats in each group. All groups except for the normal group and sham operation group were prepared as the dry eye models by using the method of ovariectomy. After one week, the rats were treated with medicines. After three months treatment, schirmer tear test (STT) and tear film breakup time (BUT) of rats were detected. Theconjunctival epithelialcells were taken to test the expressive activities of p38MAPK by immunohistochemical methodand ICAM-1 and p-p38MAPK by Western blot. Results Compared with the model group, SIT of Yangyin Runmu Pills group was increased obviously, BUT was extended obviously and the expressions of ICAM-1, p38MAPK, p-p38MAPK in conjunctival epithelial cellswere decreased evidently, the differenceswere statistically significant (P<0.01). Conclusion Yangyin Runmu Pills can improve the symptoms of ocular surface, increase the tear secretion,prolong appears broken, and down-regulatethe expression of ICAM-1, p38MAPK, p-p38MAPK in conjunctival epithelial cells, which suggests that the pathogenesis of dry eye is related with inflammation and p38MAPK signaling pathway, and Yangyin Runmu Pills have potential application fortreating dry eye.