CAJ | 학술논문

目的：研究白术七物颗粒对气阴两虚型结肠慢传输型便秘大鼠结肠组织神经元特异性烯醇化酶（neuron specific enolase，NSE）的影响，初步探讨白术七物颗粒对结肠慢传输型便秘的作用机制。方法将60只SD大鼠按照完全随机原则分为正常组、模型组、治疗组，每组各20只。正常组予以皮下注射等体积等渗生理盐水，模型组、治疗组予以皮下注射盐酸吗啡注射液2.5 mg/(kg·d)，共45 d，并同时灌服青皮（18 g）、枳壳（18 g）、附子（15 g）溶液，建立气阴两虚型结肠慢传输型便秘模型。造模成功后各组分别予以相应药物进行灌胃治疗，每天1次，连续14 d，末次给药后检测各组大鼠结肠组织中NSE的含量。结果造模前,各组大鼠粪便干重比较,差异无统计学意义；造模后，模型组、治疗组大鼠粪便干重与正常组比较明显减少，差异有统计学意义（P<0.01）；造模后,模型组、治疗组肠道炭末推进率与正常组比较明显降低，差异有统计学意义(P<0.01)；模型组与治疗组大鼠结肠组织中NSE含量明显降低，与正常组相比，差异有统计学意义(P<0.05)；治疗组与模型组相比，NSE含量相对升高，差异有统计学意义(P<0.05)。结论白术七物颗粒能有效治疗气阴两虚型结肠慢传输型便秘，其作用与提升NSE的含量关系密切。
목적：연구백술칠물과립대기음량허형결장만전수형편비대서결장조직신경원특이성희순화매（neuron specific enolase，NSE）적영향，초보탐토백술칠물과립대결장만전수형편비적작용궤제。방법장60지SD대서안조완전수궤원칙분위정상조、모형조、치료조，매조각20지。정상조여이피하주사등체적등삼생리염수，모형조、치료조여이피하주사염산마배주사액2.5 mg/(kg·d)，공45 d，병동시관복청피（18 g）、지각（18 g）、부자（15 g）용액，건립기음량허형결장만전수형편비모형。조모성공후각조분별여이상응약물진행관위치료，매천1차，련속14 d，말차급약후검측각조대서결장조직중NSE적함량。결과조모전,각조대서분편간중비교,차이무통계학의의；조모후，모형조、치료조대서분편간중여정상조비교명현감소，차이유통계학의의（P<0.01）；조모후,모형조、치료조장도탄말추진솔여정상조비교명현강저，차이유통계학의의(P<0.01)；모형조여치료조대서결장조직중NSE함량명현강저，여정상조상비，차이유통계학의의(P<0.05)；치료조여모형조상비，NSE함량상대승고，차이유통계학의의(P<0.05)。결론백술칠물과립능유효치료기음량허형결장만전수형편비，기작용여제승NSE적함량관계밀절。
〔Abstract〕 Objective To study the effect of Baizhu Qiwu Granules on neuron specific enolase (NSE) in colonic tissues of colon slow transit constipation rats with deficiency of both Qi and Yin syndrome and to explore its mechanism of action. Methods 60 SD rats were randomly divided into normal group, model group and treatment group, 20 rats in each group. The rats in normal group were given isovolumetric and isotonic normal saline in subcutaneous injections and the remaining rats in model and treatment groups were daily subcutaneous injection of 2.5mg/kg·d of morphine hydrochloride for total 45 days, and at the same time administered with Qingpi (18 g), Zhike (18 g) and Fuzi (15 g) to build thecolon slow transit constipation model rats with deficiency of both Qi and Yin syndrome.After establishing the models successfully, the rats in each group are given the corresponding drug treatment once daily for 14 days. At the final injection,the level of NSE in colonic tissues of rats was measured. Results There was no significant difference in the dry weight of the feces of the rats in each group before building. After modeling, the dry weight of the feces of rats in the model group and the treatment group were significantly decreased compared with the normal group, the differences have statistical significance (P<0.01). The rate of intestinal charcoal propulsion in the model group and the treatment group were significantly decreased compared with the normal group, the differences have statistical significance (P<0.01). The content level of NSE in colon tissue of rats in the model group and treatment group was increased compared to the normal group, the differences have statistical significance (P <0.05). The content level of NSE in colon tissue of rats in the treatment group was decreased compared to the normal group, the differences have statistical significance (P<0.05). Conclusion Baizhu Qiwu Granules can effectively treat both Qi and Yin type of colonic slow transit constipation,which may be related with the elevated NSE level.