江西农业大学学报
江西農業大學學報
강서농업대학학보
Acta Agriculturae Universitatis Jiangxiensis(Natural Sciences Edition)
2015年
5期
843-848,858
,共7页
林静雯%李莹%罗洁文%周垂帆%刘爱琴
林靜雯%李瑩%囉潔文%週垂帆%劉愛琴
림정문%리형%라길문%주수범%류애금
草甘膦%杉木种子%发芽率%抗氧化酶%叶绿素
草甘膦%杉木種子%髮芽率%抗氧化酶%葉綠素
초감련%삼목충자%발아솔%항양화매%협록소
glyphosate%Chinese fir%germination rate%antioxidant enzyme%chlorophyll
选取南方重要速生造林树种——杉木为研究对象,采用砂培法即在石英砂中添加不同浓度草甘膦(0、2、5、10、50 mg/kg)进行杉木种子发芽试验,研究林业上广泛使用的除草剂草甘膦对杉木种子萌发及幼苗生长影响,为评估林业除草剂对人工林环境的生态风险提供科学依据.结果表明,随着草甘膦浓度增加,杉木种子发芽率、萌发后幼苗的根长、茎长、叶长和鲜质量受到的抑制作用越显著,且根长对草甘膦的反应最敏感.此外,草甘膦浓度升高使杉木幼苗SOD活性下降,CAT活性上升,POD活性则呈现先升后降的趋势,同时造成MDA含量上升和叶绿素含量下降.因此认为草甘膦对杉木种子发芽具有抑制效应,并导致幼苗抗氧化酶系统功能紊乱,造成细胞氧化损伤以及叶绿素合成受阻.
選取南方重要速生造林樹種——杉木為研究對象,採用砂培法即在石英砂中添加不同濃度草甘膦(0、2、5、10、50 mg/kg)進行杉木種子髮芽試驗,研究林業上廣汎使用的除草劑草甘膦對杉木種子萌髮及幼苗生長影響,為評估林業除草劑對人工林環境的生態風險提供科學依據.結果錶明,隨著草甘膦濃度增加,杉木種子髮芽率、萌髮後幼苗的根長、莖長、葉長和鮮質量受到的抑製作用越顯著,且根長對草甘膦的反應最敏感.此外,草甘膦濃度升高使杉木幼苗SOD活性下降,CAT活性上升,POD活性則呈現先升後降的趨勢,同時造成MDA含量上升和葉綠素含量下降.因此認為草甘膦對杉木種子髮芽具有抑製效應,併導緻幼苗抗氧化酶繫統功能紊亂,造成細胞氧化損傷以及葉綠素閤成受阻.
선취남방중요속생조림수충——삼목위연구대상,채용사배법즉재석영사중첨가불동농도초감련(0、2、5、10、50 mg/kg)진행삼목충자발아시험,연구임업상엄범사용적제초제초감련대삼목충자맹발급유묘생장영향,위평고임업제초제대인공림배경적생태풍험제공과학의거.결과표명,수착초감련농도증가,삼목충자발아솔、맹발후유묘적근장、경장、협장화선질량수도적억제작용월현저,차근장대초감련적반응최민감.차외,초감련농도승고사삼목유묘SOD활성하강,CAT활성상승,POD활성칙정현선승후강적추세,동시조성MDA함량상승화협록소함량하강.인차인위초감련대삼목충자발아구유억제효응,병도치유묘항양화매계통공능문란,조성세포양화손상이급협록소합성수조.
Glyphosate is one of the most widely used herbicides in forestry and agricultural production,but it may affect the non-target organisms in soils.Therefore,a laboratory experiment was conducted to investigate the effects of glyphosate on seed germination and seedling growth of Chinese fir which is one of the important fast-growing species in the south and assess the ecological risk of glyphosate to artificial forest.The results showed that,the seed germination rate as well as the root length,stem length,leaf length and fresh weight of seedlings decreased significantly with the increase of glyphosate.The root length was more sensitive to glypho-sate than other indexes.In addition,with the increase of glyphosate,the activity of SOD and chlorophyll content in Chinese fir seedlings decreased,while the activity of CAT and MDA content increased.The activity of POD presented earlier increased and then declined.There is an inhibitory effect of glyphosate on Chinese fir seeds and seedlings,which led to antioxidant enzyme dysfunction,oxidative damage of cell and declined chlorophyll synthesis.