CAJ | 학술논문

以紫红肉火龙果为试验材料,对影响有机酸分离的主要因素进行研究,最终建立了运用反相高效液相色谱法(RP-HPLC)同时快速测定火龙果果实中草酸、酒石酸、苹果酸、乙酸、乳酸、柠檬酸、琥珀酸7种有机酸及Vc含量的方法.采用色谱条件为:柱温30 ℃,流速1 mL/min,色谱柱为Kromasil柱(250 mm×4.6 mm),波长215 nm,缓冲液0.05 mol/L H3 PO4-Na2 HPO4;测定结果显示,供试火龙果含有7种有机酸和Vc,苹果酸是其最主要的有机酸、其次是柠檬酸.此方法可以在10 min内完成1次测定,具有分析速度快、灵敏度高、重复性好等优点,为果实中有机酸及Vc的测定及相关代谢研究提供参考.
이자홍육화룡과위시험재료,대영향유궤산분리적주요인소진행연구,최종건립료운용반상고효액상색보법(RP-HPLC)동시쾌속측정화룡과과실중초산、주석산、평과산、을산、유산、저몽산、호박산7충유궤산급Vc함량적방법.채용색보조건위:주온30 ℃,류속1 mL/min,색보주위Kromasil주(250 mm×4.6 mm),파장215 nm,완충액0.05 mol/L H3 PO4-Na2 HPO4;측정결과현시,공시화룡과함유7충유궤산화Vc,평과산시기최주요적유궤산、기차시저몽산.차방법가이재10 min내완성1차측정,구유분석속도쾌、령민도고、중복성호등우점,위과실중유궤산급Vc적측정급상관대사연구제공삼고.
The main factors that affect the separation of organic acids were studied,and a method for sim-ultaneous and rapid determination of purple red flesh dragon fruit in oxalic acid,tartaric acid,malic acid,acetic acid,lactic acid,citric acid,succinic acid and Vc content with reverse-phase high performance liquid chroma-tography(RP-HPLC) was established.The chromatographic conditions are as follows:the column temperature was 30 ℃,the flow rate was 1 mL/min,the wavelength was 215 nm,the buffer was 0.05 mol/L H3 PO4-Na2HPO4 and a Kromasil column (250 mm× 4.6 mm) was adopted.The results showed that malic acid was the most important organic acid,the next was citric acid.Because this method can complete a determination within10 min.So it has the advantages of fast,high sensitivity and good repeatability,it is a high reference to the de-termination and research of metabolism of organic acid and Vc in fruit.