江西农业大学学报
江西農業大學學報
강서농업대학학보
Acta Agriculturae Universitatis Jiangxiensis(Natural Sciences Edition)
2015年
5期
793-797
,共5页
肖旭峰%解庆娓%范淑英%刘威砾%黄敏
肖旭峰%解慶娓%範淑英%劉威礫%黃敏
초욱봉%해경미%범숙영%류위력%황민
Cd%Pb%复合污染%芹菜%抗氧化酶
Cd%Pb%複閤汙染%芹菜%抗氧化酶
Cd%Pb%복합오염%근채%항양화매
Cd%Pb%compound pollution%celery (Apium graveolens L.)%antioxidant enzymes
采用外源添加重金属和营养液水培试验研究了重金属镉(Cd)、铅(Pb)单一及复合污染对芹菜生长周期的生长及根系活力、脯氨酸(Pro)含量、抗氧化酶活性的影响.结果表明:(1) 6~8 mg/L Cd和60~80 mg/L Pb单一及复合处理对芹菜全生长期内生物量、株高、根系活力、脯氨酸及抗氧化酶活性(SOD、POD)的影响均达到极显著性效应(P<0.01);(2)低浓度Cd(≤2 mg/L)、Pb(≤20 mg/L)及Cd-Pb复合(≤(2+20)mg/L)条件下,镉、铅污染对芹菜植株生物量、株高具有促进效应,而高浓度、长时间胁迫时,植株生物量及受到明显的抑制(P<0.01);(3)Cd、Pb单一及复合胁迫对芹菜植株生物量、株高、根系活力、脯氨酸及抗氧化酶活性(SOD、POD)的影响毒性由大到小依次均为Cd-Pb交互、Cd、Pb、CK;(4)低浓度、短时间重金属处理对芹菜根系活力的影响不显著;但高浓度、长时间处理时,根系活力变化呈现极显著负相关性(P<0.01);(5)Cd、Pb对芹菜叶POD和SOD活性的影响相似,都表现为低浓度下促进酶活性,而高浓度下抑制酶活性;(6)重金属浓度越高,芹菜叶片内诱导合成脯氨酸越多,这可在一定程度上缓解重金属污染对芹菜植株的毒害作用.
採用外源添加重金屬和營養液水培試驗研究瞭重金屬鎘(Cd)、鉛(Pb)單一及複閤汙染對芹菜生長週期的生長及根繫活力、脯氨痠(Pro)含量、抗氧化酶活性的影響.結果錶明:(1) 6~8 mg/L Cd和60~80 mg/L Pb單一及複閤處理對芹菜全生長期內生物量、株高、根繫活力、脯氨痠及抗氧化酶活性(SOD、POD)的影響均達到極顯著性效應(P<0.01);(2)低濃度Cd(≤2 mg/L)、Pb(≤20 mg/L)及Cd-Pb複閤(≤(2+20)mg/L)條件下,鎘、鉛汙染對芹菜植株生物量、株高具有促進效應,而高濃度、長時間脅迫時,植株生物量及受到明顯的抑製(P<0.01);(3)Cd、Pb單一及複閤脅迫對芹菜植株生物量、株高、根繫活力、脯氨痠及抗氧化酶活性(SOD、POD)的影響毒性由大到小依次均為Cd-Pb交互、Cd、Pb、CK;(4)低濃度、短時間重金屬處理對芹菜根繫活力的影響不顯著;但高濃度、長時間處理時,根繫活力變化呈現極顯著負相關性(P<0.01);(5)Cd、Pb對芹菜葉POD和SOD活性的影響相似,都錶現為低濃度下促進酶活性,而高濃度下抑製酶活性;(6)重金屬濃度越高,芹菜葉片內誘導閤成脯氨痠越多,這可在一定程度上緩解重金屬汙染對芹菜植株的毒害作用.
채용외원첨가중금속화영양액수배시험연구료중금속력(Cd)、연(Pb)단일급복합오염대근채생장주기적생장급근계활력、포안산(Pro)함량、항양화매활성적영향.결과표명:(1) 6~8 mg/L Cd화60~80 mg/L Pb단일급복합처리대근채전생장기내생물량、주고、근계활력、포안산급항양화매활성(SOD、POD)적영향균체도겁현저성효응(P<0.01);(2)저농도Cd(≤2 mg/L)、Pb(≤20 mg/L)급Cd-Pb복합(≤(2+20)mg/L)조건하,력、연오염대근채식주생물량、주고구유촉진효응,이고농도、장시간협박시,식주생물량급수도명현적억제(P<0.01);(3)Cd、Pb단일급복합협박대근채식주생물량、주고、근계활력、포안산급항양화매활성(SOD、POD)적영향독성유대도소의차균위Cd-Pb교호、Cd、Pb、CK;(4)저농도、단시간중금속처리대근채근계활력적영향불현저;단고농도、장시간처리시,근계활력변화정현겁현저부상관성(P<0.01);(5)Cd、Pb대근채협POD화SOD활성적영향상사,도표현위저농도하촉진매활성,이고농도하억제매활성;(6)중금속농도월고,근채협편내유도합성포안산월다,저가재일정정도상완해중금속오염대근채식주적독해작용.
The influences of Cd,Pb single and combined pollution on biomass,plant height,root activi-ties,proline (Pro),antioxidant enzyme superoxide dismutase (SOD),peroxidase (POD) of celery(Apium graveolens) in nutrient solution were studied,which supplied reference for ensuring vegetable production safety.The results showed:(1) During the whole growth cycle of celery,the influences of 6-8 mg/L Cd,60-80 mg/L Pb single and interactive stress on biomass,plant height,root activities,proline and activities of SOD,POD in the leaves were very significant (P<0.01).(2) The biomass and plant height of celery were boosted under low contents of Cd (≤2 mg/L),Pb (≤20 mg/L) and Cd-Pb interaction (≤(2+20)mg/L),and were restrain-ed under high concentrations and long time (P<0.01).(3) The effects of Cd,Pb pollution on biomass,plant height,root activities,proline and antioxidant enzyme system (SOD,POD) of celery showed the order:Cd-Pb combined pollution>Cd>Pb>CK.(4) Root activity was non-significantly affected under low concentrations and short time,but was significantly negative correlated with heavy metal stress under high concentrations and long time (P<0.01).(5) The effects of Cd and Pb on POD and SOD activities in leaf extracts of celery were simi-lar.The enzyme activities were stimulated under lower metal ion concentrations but were inhabited under higher concentrations.(6) The higher heavy metal concentrations,the higher Pro content,which can alleviate Cd and Pb toxicity to celery seedlings.
