陕西科技大学学报（自然科学版）
陝西科技大學學報（自然科學版）
협서과기대학학보（자연과학판）
Journal of Shaanxi University of Science and Technology (Natural Science Edition)
2015年
6期
121-126
,共6页
冠突散囊菌%液体发酵%黄色素%微波辅助提取
冠突散囊菌%液體髮酵%黃色素%微波輔助提取
관돌산낭균%액체발효%황색소%미파보조제취
Eurotium cristatum%liquid fermentation%pigments%microwave-assisted extrac-tion
基于Box‐Behnken响应面试验设计确定了冠突散囊菌液体发酵菌丝体黄色素的最佳提取条件．采用单因素法确定影响冠突散囊菌黄色素得率的主要因素，即料液比、提取温度和微波功率，进一步利用Box‐Behnken模型对其进行响应面优化．结果表明：影响黄色素提取效果的最主要因素是料液比和提取温度，其次是微波功率．确定最佳提取工艺为料液比1∶38（w／v ），提取温度56℃，微波功率457 W ．该条件下黄色素得率10．08％．实测结果与响应面拟合所得方程的预测值符合良好，采用响应面法对微波辅助提取冠突散囊菌黄色素的提取条件优化合理．
基于Box‐Behnken響應麵試驗設計確定瞭冠突散囊菌液體髮酵菌絲體黃色素的最佳提取條件．採用單因素法確定影響冠突散囊菌黃色素得率的主要因素，即料液比、提取溫度和微波功率，進一步利用Box‐Behnken模型對其進行響應麵優化．結果錶明：影響黃色素提取效果的最主要因素是料液比和提取溫度，其次是微波功率．確定最佳提取工藝為料液比1∶38（w／v ），提取溫度56℃，微波功率457 W ．該條件下黃色素得率10．08％．實測結果與響應麵擬閤所得方程的預測值符閤良好，採用響應麵法對微波輔助提取冠突散囊菌黃色素的提取條件優化閤理．
기우Box‐Behnken향응면시험설계학정료관돌산낭균액체발효균사체황색소적최가제취조건．채용단인소법학정영향관돌산낭균황색소득솔적주요인소，즉료액비、제취온도화미파공솔，진일보이용Box‐Behnken모형대기진행향응면우화．결과표명：영향황색소제취효과적최주요인소시료액비화제취온도，기차시미파공솔．학정최가제취공예위료액비1∶38（w／v ），제취온도56℃，미파공솔457 W ．해조건하황색소득솔10．08％．실측결과여향응면의합소득방정적예측치부합량호，채용향응면법대미파보조제취관돌산낭균황색소적제취조건우화합리．
The conditions for the aqueous extraction of pigments from Eurotium cristatum were optimized with Box‐Behnken design under response surface methodology .The major ex‐traction factors of pigments from Eurotium cristatum were obtained by single factor experi‐ments .And they were solvent‐solid ratio ,extraction temperature ,microwave power on yield of extracted for pigments from by Box‐Behnken design .The optimal conditions of micro‐wave‐assisted extraction for pigments were:solvent‐solid ratio 1∶38(w/v) ,extraction tem‐perature 56 ℃ ,microwave power 457 W .Under these conditions ,average yield of pigments was 10 .08% .And the measured value was consistent with the predicated value ,which indi‐cated the applicability of RSM to the optimization of extraction of pigments from Eurotium cristatum .
