CAJ | 학술논문

为了获得适用于发酵生产Nisin的海藻酸钠（A ）‐壳聚糖（C ）‐海藻酸钠（A ）乳链球菌6032的液芯微囊，以其发酵液吸光度值OD600、Nisin的效价为综合指标，探讨了影响ACA乳链球菌6032液芯微囊发酵性能的制备工艺条件．在单因素实验的基础上采用Box‐Behnken模型响应面设计，建立了乳酸菌ACA液芯微囊制备的三元二次回归模型，并确定了其制备因素的最佳水平值为壳聚糖溶解时间2 h、搅拌速度236 r／m in、制备温度33．7℃、滴加速度4．9 mL／min ．将此条件下制得的乳酸菌ACA液芯微囊发酵生产Nisin ，吸光度值OD600为0．181， Nisin效价为5242 IU／mL ，该结果与模型预测值拟合良好，说明响应面分析法优化该实验是有效的．
위료획득괄용우발효생산Nisin적해조산납（A ）‐각취당（C ）‐해조산납（A ）유련구균6032적액심미낭，이기발효액흡광도치OD600、Nisin적효개위종합지표，탐토료영향ACA유련구균6032액심미낭발효성능적제비공예조건．재단인소실험적기출상채용Box‐Behnken모형향응면설계，건립료유산균ACA액심미낭제비적삼원이차회귀모형，병학정료기제비인소적최가수평치위각취당용해시간2 h、교반속도236 r／m in、제비온도33．7℃、적가속도4．9 mL／min ．장차조건하제득적유산균ACA액심미낭발효생산Nisin ，흡광도치OD600위0．181， Nisin효개위5242 IU／mL ，해결과여모형예측치의합량호，설명향응면분석법우화해실험시유효적．
In order to obtain the ACA liquid core microcapsules of lactococcus lactis 6032 for Nisin fermentation ,absorbance value of OD600 and titer unit of Nisin in the broth as compre‐hensive indicators ,the liquid core microcapsules preparation conditions affect on fermentation of L actobacillus was discussed .Based on one‐factor experimental design ,the quadratic model was founded by the Box‐Behnken response surface model .The optimal value of each prepara‐tion condition were chitosan dissolution time 2 h ,stirring speed 236 r/min ,temperature 33 .7℃ ,dropping rate 4 .9 mL/min .Under these conditions ,the absorbance value of OD600 was 0 .181 and the titer unit of Nisin was 5 242 IU/mL ,which is consistent with the predicted value .These showed that it is effective for optimizing preparation conditions by response sur‐face methodology .