中医临床研究
中醫臨床研究
중의림상연구
Clinical Journal of Chinese Medicine
2015年
29期
1-2,5
,共3页
补脑止痫散%戊四氮%癫痫大鼠%γ-氨基丁酸%氨基丁酸受体亚单位α
補腦止癇散%戊四氮%癲癇大鼠%γ-氨基丁痠%氨基丁痠受體亞單位α
보뇌지간산%무사담%전간대서%γ-안기정산%안기정산수체아단위α
Bunao Zhixian San%Pentylenetetrazole%Epileptic rats%γ-GABA%GABAα
目的：揭示补脑止痫散对戊四氮（PTZ）慢性点燃癫痫大鼠模型γ-氨基丁酸（GABA）及其受体亚单位α（GABAα）表达的影响。方法：①选取60只健康SD大鼠，分成6组（空白对照组、癫痫模型组、丙戊酸钠组、补脑止痫散小剂量组、补脑止痫散中剂量组、补脑止痫散高剂量组）。②采用连续小剂量腹腔注射戊四氮（PTZ）的方法，建立癫痫大鼠模型（慢性点燃）。凡是获得III级以上发作后，均停止给药。后采用免疫组织化学法，检测各组大鼠海马区GABA及其GABAα的表达。结果：除空白对照组无癫痫样发作外，其余各组都出现Racine分级中的III-IV痫样发作表现。通过免疫组织化学法，检测各组大鼠海马区GABA及其GABAα的表达。丙戊酸钠组、补脑止痫散高剂量组GABA及GABAα表达明显上调，与模型组比较，差异显著，具有统计学意义（P<0.05）。结论：补脑止痫散对戊四氮慢性点燃癫痫大鼠模型海马区内抑制性递质及受体有明显的上调作用，从而起到保护脑组织、缓解癫痫症状，进而减轻由癫痫导致的脑损伤的作用。
目的：揭示補腦止癇散對戊四氮（PTZ）慢性點燃癲癇大鼠模型γ-氨基丁痠（GABA）及其受體亞單位α（GABAα）錶達的影響。方法：①選取60隻健康SD大鼠，分成6組（空白對照組、癲癇模型組、丙戊痠鈉組、補腦止癇散小劑量組、補腦止癇散中劑量組、補腦止癇散高劑量組）。②採用連續小劑量腹腔註射戊四氮（PTZ）的方法，建立癲癇大鼠模型（慢性點燃）。凡是穫得III級以上髮作後，均停止給藥。後採用免疫組織化學法，檢測各組大鼠海馬區GABA及其GABAα的錶達。結果：除空白對照組無癲癇樣髮作外，其餘各組都齣現Racine分級中的III-IV癇樣髮作錶現。通過免疫組織化學法，檢測各組大鼠海馬區GABA及其GABAα的錶達。丙戊痠鈉組、補腦止癇散高劑量組GABA及GABAα錶達明顯上調，與模型組比較，差異顯著，具有統計學意義（P<0.05）。結論：補腦止癇散對戊四氮慢性點燃癲癇大鼠模型海馬區內抑製性遞質及受體有明顯的上調作用，從而起到保護腦組織、緩解癲癇癥狀，進而減輕由癲癇導緻的腦損傷的作用。
목적：게시보뇌지간산대무사담（PTZ）만성점연전간대서모형γ-안기정산（GABA）급기수체아단위α（GABAα）표체적영향。방법：①선취60지건강SD대서，분성6조（공백대조조、전간모형조、병무산납조、보뇌지간산소제량조、보뇌지간산중제량조、보뇌지간산고제량조）。②채용련속소제량복강주사무사담（PTZ）적방법，건립전간대서모형（만성점연）。범시획득III급이상발작후，균정지급약。후채용면역조직화학법，검측각조대서해마구GABA급기GABAα적표체。결과：제공백대조조무전간양발작외，기여각조도출현Racine분급중적III-IV간양발작표현。통과면역조직화학법，검측각조대서해마구GABA급기GABAα적표체。병무산납조、보뇌지간산고제량조GABA급GABAα표체명현상조，여모형조비교，차이현저，구유통계학의의（P<0.05）。결론：보뇌지간산대무사담만성점연전간대서모형해마구내억제성체질급수체유명현적상조작용，종이기도보호뇌조직、완해전간증상，진이감경유전간도치적뇌손상적작용。
Objectives: To study the effects of Bunao Zhixian San on expression ofγ-aminobutyric acid (GABA) and GABAα in epilepsy rats. Methods: 60 SD rats were randomly divided into 6 groups, control group, epileptic rat model group, sodium valproate group, low dose of Bunao Zhixian San therapy group, middle dose of Bunao Zhixian San therapy group and high dose of Bunao Zhixian San therapy group. Rat kindling model of epilepsy was established by PTZ injection. By immunohistochemistry method, the protein expression of GABA and GABAα in hippocampus of rats was detected. Results: The occurrence and seizure level were evaluated by Racine’5 methods. The rats with III to V seizure level were chosen for the following studies. The expression of GABA and GABAα were increased significantly in the sodium valproate group and the high dose of Bunao Zhixian San therapy group. There were significant difference compared with model group (P<0.05). Conclusions: Bunao Zhixian San has significantly increased role in excitatory neurotransmitter and receptor, to protect the brain tissue, alleviate the symptoms of epilepsy, and reduce the damage caused by epilepsy.
