河南科学
河南科學
하남과학
Henan Science
2015年
10期
1750-1755
,共6页
程雁%王景冒%张岩%杨潜龙%邱立友
程雁%王景冒%張巖%楊潛龍%邱立友
정안%왕경모%장암%양잠룡%구립우
双孢蘑菇%1-氨基环丙烷-1-羧酸脱氨酶%覆土%蛭石
雙孢蘑菇%1-氨基環丙烷-1-羧痠脫氨酶%覆土%蛭石
쌍포마고%1-안기배병완-1-최산탈안매%복토%질석
Agaricus bisporus%1-aminocyclopropane-1-carboxylic acid deaminase%casing soil%vermiculite
比较了6种覆土处理对双孢蘑菇产量的影响. 6种覆土处理分别是常规覆土、常规覆土+恶臭假单胞菌UW4菌剂(用量为覆土干重的2%)、灭菌常规覆土、灭菌常规覆土+UW4菌剂(用量为覆土干重的2%)、灭菌蛭石和灭菌蛭石+UW4菌剂(用量为蛭石干重的2%). UW4是常用的植物根际促生细菌1-氨基环丙烷-1-羧酸(ACC)脱氨酶产生菌. 结果表明,灭菌蛭石+UW4菌剂处理出菇最早,产量最高,无杂菌污染,出菇比其他处理提早1~8 d,比常规覆土提早3 d,第一潮和第二潮菇产量之和比其他处理提高19.8%~115%,比常规覆土提高35.1%. 采收二潮菇后,不同处理覆土材料中细菌和ACC脱氨酶产生菌的数量均与菇产量呈显著的正相关. 本研究结果提示,灭菌蛭石中添加ACC脱氨酶产生菌剂替代传统覆土是实现双孢蘑菇绿色、高效生产的有效方式.
比較瞭6種覆土處理對雙孢蘑菇產量的影響. 6種覆土處理分彆是常規覆土、常規覆土+噁臭假單胞菌UW4菌劑(用量為覆土榦重的2%)、滅菌常規覆土、滅菌常規覆土+UW4菌劑(用量為覆土榦重的2%)、滅菌蛭石和滅菌蛭石+UW4菌劑(用量為蛭石榦重的2%). UW4是常用的植物根際促生細菌1-氨基環丙烷-1-羧痠(ACC)脫氨酶產生菌. 結果錶明,滅菌蛭石+UW4菌劑處理齣菇最早,產量最高,無雜菌汙染,齣菇比其他處理提早1~8 d,比常規覆土提早3 d,第一潮和第二潮菇產量之和比其他處理提高19.8%~115%,比常規覆土提高35.1%. 採收二潮菇後,不同處理覆土材料中細菌和ACC脫氨酶產生菌的數量均與菇產量呈顯著的正相關. 本研究結果提示,滅菌蛭石中添加ACC脫氨酶產生菌劑替代傳統覆土是實現雙孢蘑菇綠色、高效生產的有效方式.
비교료6충복토처리대쌍포마고산량적영향. 6충복토처리분별시상규복토、상규복토+악취가단포균UW4균제(용량위복토간중적2%)、멸균상규복토、멸균상규복토+UW4균제(용량위복토간중적2%)、멸균질석화멸균질석+UW4균제(용량위질석간중적2%). UW4시상용적식물근제촉생세균1-안기배병완-1-최산(ACC)탈안매산생균. 결과표명,멸균질석+UW4균제처리출고최조,산량최고,무잡균오염,출고비기타처리제조1~8 d,비상규복토제조3 d,제일조화제이조고산량지화비기타처리제고19.8%~115%,비상규복토제고35.1%. 채수이조고후,불동처리복토재료중세균화ACC탈안매산생균적수량균여고산량정현저적정상관. 본연구결과제시,멸균질석중첨가ACC탈안매산생균제체대전통복토시실현쌍포마고록색、고효생산적유효방식.
In this study,the effects of the six casing treatments on the yield of the button mushroom were compared. The six casing treatments were that soil casing,soil+Pseudomonas putida UW4 bacterial agent(2%of the soil dry weight),sterilized soil,sterilized soil+UW4 bacterial agent(2% of the soil dry weight),sterilized vermiculite,and sterilized vermiculite+UW4 bacterial agent(2% of the vermiculite dry weight),respectively. Pseudomonas putida UW4 is commonly used as plant growth-promoting rhizobacteria and 1-aminocyclopropane-1-carboxylic acid(ACC)deaminase producing bacteria. The results showed that the fruiting bodies emerged earliest (the duration from casing to primordium initiation was earlier 1-8 d)and the yield of the first and second crops was the highest(higher 19.8%to 115%)with not any contamination by casing sterilized vermiculite+UW4 compared to the other casing treatments. In details,casing of sterilized vermiculite+UW4 the fruiting bodies emerged earlier 3 d and the yield of the first and second crops was higher 35.1%than that of soil casing. The number of bacteria and ACC deaminase producing bacteria in the casing material after harvest two flush mushrooms and the yield of the first and second crops showed significant positive correlation,respectively. The results of this study suggest that it is a facility procedure for green and efficient production of the button mushroom by using sterilized vermiculite adding ACC deaminase producing bacteria agent instead of the traditional soil casing.