海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
21期
3127-3130
,共4页
埃兹蛋白%基因p16%宫颈癌%宫颈柱状上皮异位
埃玆蛋白%基因p16%宮頸癌%宮頸柱狀上皮異位
애자단백%기인p16%궁경암%궁경주상상피이위
Ezrin%p16%Cervical cancer%Cervical columnar epithelium ectopy
目的 探讨埃兹蛋白(Ezrin)和p16基因在宫颈癌和宫颈柱状上皮异位组织中的表达及其临床意义.方法 采用实时逆转录-聚合酶链反应(RT-PCR)技术检测39例宫颈癌患者和35例宫颈柱状上皮异位患者组织Ezrin mRNA和p16 mRNA的表达,并选取正常宫颈组织作为对照组,比较分析以上指标的变化.结果 Ezrin mRNA和p16 mRNA在宫颈癌组、宫颈柱状上皮异位组及对照组中的表达率分别为89.7%、22.8%、21.0%和94.8%、22.8%、5.2%.Ezrin mRNA在宫颈癌组织中的表达率显著高于宫颈柱状上皮异位组及对照组(P<0.01),在宫颈柱状上皮异位与对照组中Ezrin mRNA表达率比较差异无统计学意义(P>0.05).p16 mRNA在宫颈癌与宫颈柱状上皮异位组织中的表达率显著高于对照组(P<0.01),在宫颈癌组织的表达率高于宫颈柱状上皮异位组织,差异均有显著统计学意义(P<0.01).Ezrin mRNA和p16 mRNA分别在宫颈癌组、宫颈柱状上皮异位组及对照组中的表达量分别为(0.82±0.17)、(0.25±0.06)、(0.23±0.07)和(0.77±0.12)、(0.35±0.07)、(0.22±0.05).Ezrin mRNA和p16 mRNA在宫颈癌组中的表达量具有相关性(r=0.539,P<0.01).结论 检测组织Ezrin和p16基因表达可应用于宫颈癌和宫颈柱状上皮异位的鉴别诊断,并为宫颈癌的早期发现提供依据.
目的 探討埃玆蛋白(Ezrin)和p16基因在宮頸癌和宮頸柱狀上皮異位組織中的錶達及其臨床意義.方法 採用實時逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測39例宮頸癌患者和35例宮頸柱狀上皮異位患者組織Ezrin mRNA和p16 mRNA的錶達,併選取正常宮頸組織作為對照組,比較分析以上指標的變化.結果 Ezrin mRNA和p16 mRNA在宮頸癌組、宮頸柱狀上皮異位組及對照組中的錶達率分彆為89.7%、22.8%、21.0%和94.8%、22.8%、5.2%.Ezrin mRNA在宮頸癌組織中的錶達率顯著高于宮頸柱狀上皮異位組及對照組(P<0.01),在宮頸柱狀上皮異位與對照組中Ezrin mRNA錶達率比較差異無統計學意義(P>0.05).p16 mRNA在宮頸癌與宮頸柱狀上皮異位組織中的錶達率顯著高于對照組(P<0.01),在宮頸癌組織的錶達率高于宮頸柱狀上皮異位組織,差異均有顯著統計學意義(P<0.01).Ezrin mRNA和p16 mRNA分彆在宮頸癌組、宮頸柱狀上皮異位組及對照組中的錶達量分彆為(0.82±0.17)、(0.25±0.06)、(0.23±0.07)和(0.77±0.12)、(0.35±0.07)、(0.22±0.05).Ezrin mRNA和p16 mRNA在宮頸癌組中的錶達量具有相關性(r=0.539,P<0.01).結論 檢測組織Ezrin和p16基因錶達可應用于宮頸癌和宮頸柱狀上皮異位的鑒彆診斷,併為宮頸癌的早期髮現提供依據.
목적 탐토애자단백(Ezrin)화p16기인재궁경암화궁경주상상피이위조직중적표체급기림상의의.방법 채용실시역전록-취합매련반응(RT-PCR)기술검측39례궁경암환자화35례궁경주상상피이위환자조직Ezrin mRNA화p16 mRNA적표체,병선취정상궁경조직작위대조조,비교분석이상지표적변화.결과 Ezrin mRNA화p16 mRNA재궁경암조、궁경주상상피이위조급대조조중적표체솔분별위89.7%、22.8%、21.0%화94.8%、22.8%、5.2%.Ezrin mRNA재궁경암조직중적표체솔현저고우궁경주상상피이위조급대조조(P<0.01),재궁경주상상피이위여대조조중Ezrin mRNA표체솔비교차이무통계학의의(P>0.05).p16 mRNA재궁경암여궁경주상상피이위조직중적표체솔현저고우대조조(P<0.01),재궁경암조직적표체솔고우궁경주상상피이위조직,차이균유현저통계학의의(P<0.01).Ezrin mRNA화p16 mRNA분별재궁경암조、궁경주상상피이위조급대조조중적표체량분별위(0.82±0.17)、(0.25±0.06)、(0.23±0.07)화(0.77±0.12)、(0.35±0.07)、(0.22±0.05).Ezrin mRNA화p16 mRNA재궁경암조중적표체량구유상관성(r=0.539,P<0.01).결론 검측조직Ezrin화p16기인표체가응용우궁경암화궁경주상상피이위적감별진단,병위궁경암적조기발현제공의거.
Objective To explore the expression and clinical significance of Ezrin and p16 in tissues for cer-vical cancer and cervical columnar epithelium ectopy. Methods The expression of Ezrin mRNA and p16 mRNA were detected with the polymerase reaction technology in the tissues of 39 patients with cervical cancer (group A) and 35 patients with cervical columnar epithelium ectopy (group B). Normal cervical tissues were selected as the control group. The changes of above indicators were compared. Results The expression rates of Ezrin mRNA and p16 mRNA in group A, group B and the control group were 89.7%, 22.8%, 21.0%and 94.8%, 22.8%, 5.2%, respectively. The expression rates of Ezrin mRNA in group A was significantly higher than the other two groups (P<0.01), which showed no statistsically significantly differnece between group B and the control group (P>0.05). The expression rates of p16 mRNA in group A and group B were signifiantly higher than that in the control group (P<0.01), and that in group A was higher than that in group B (P<0.01). The expression quantities of Ezrin mRNA and p16 mRNA in group A, group B, the control group were (0.82±0.17), (0.25±0.06), (0.23±0.07) and (0.77±0.12), (0.35±0.07), (0.22±0.05), respectively. Ex-pression of Ezrin mRNA and p16 mRNA were corelated in group A (r=0.539, P<0.01). Conclusion Testing the expres-sion of Ezrin and p16 in tissues can be used in the differential diagnosis between cervical cancer and cervical colum-nar epithelium ectopy, which provides basis for the early discovery of cervical cancer.
