水电与新能源
水電與新能源
수전여신능원
Hydropower And Nwe Energy
2015年
11期
1-5
,共5页
包洪福%孙志禹%刁晓君%李一葳
包洪福%孫誌禹%刁曉君%李一葳
포홍복%손지우%조효군%리일위
长江流域%湖泊%沉积物%水华生消过程%酶活性
長江流域%湖泊%沉積物%水華生消過程%酶活性
장강류역%호박%침적물%수화생소과정%매활성
Yangtze River basin%lakes%sediment%outbreak and perishing of water bloom%enzyme activity
生物酶对矿质元素的生物地球化学循环有重要作用,但酶对环境的敏感性导致其活性易受外界因素影响。很多研究关注了富营养化对沉积物酶活性的影响,但却鲜见水华生消过程对沉积物生物酶活性影响的报道。本研究以长江流域典型湖泊———巢湖为对象,于水华生消不同时段采集沉积物和水体样品,分析沉积物水解酶和氧化还原酶活性随时间的变化。结果表明,巢湖水华形成期为5月15日-6月20日,持续期为6月20日-9月5日,之后进入水华消亡期。除部分时段的碱性磷酸酶外,水华区沉积物的脲酶、过氧化氢酶、多酚氧化酶活性均高于非水华区,水华区环境因子变化大导致酶活性的影响因素多样化,而非水华区环境因子变化相对较小,2种水解酶活性主要受沉积物pH值和底物含量影响,而2种氧化还原酶活性受沉积物TOC含量影响显著。从水华生消过程看,水华区脲酶、碱性磷酸酶活性变化明显大于非水华区,过氧化氢酶和多酚氧化酶也呈现类似的变化趋势。本研究表明,水华及其生消过程对沉积物生物酶的活性有影响,但影响大小与酶的种类有关。
生物酶對礦質元素的生物地毬化學循環有重要作用,但酶對環境的敏感性導緻其活性易受外界因素影響。很多研究關註瞭富營養化對沉積物酶活性的影響,但卻鮮見水華生消過程對沉積物生物酶活性影響的報道。本研究以長江流域典型湖泊———巢湖為對象,于水華生消不同時段採集沉積物和水體樣品,分析沉積物水解酶和氧化還原酶活性隨時間的變化。結果錶明,巢湖水華形成期為5月15日-6月20日,持續期為6月20日-9月5日,之後進入水華消亡期。除部分時段的堿性燐痠酶外,水華區沉積物的脲酶、過氧化氫酶、多酚氧化酶活性均高于非水華區,水華區環境因子變化大導緻酶活性的影響因素多樣化,而非水華區環境因子變化相對較小,2種水解酶活性主要受沉積物pH值和底物含量影響,而2種氧化還原酶活性受沉積物TOC含量影響顯著。從水華生消過程看,水華區脲酶、堿性燐痠酶活性變化明顯大于非水華區,過氧化氫酶和多酚氧化酶也呈現類似的變化趨勢。本研究錶明,水華及其生消過程對沉積物生物酶的活性有影響,但影響大小與酶的種類有關。
생물매대광질원소적생물지구화학순배유중요작용,단매대배경적민감성도치기활성역수외계인소영향。흔다연구관주료부영양화대침적물매활성적영향,단각선견수화생소과정대침적물생물매활성영향적보도。본연구이장강류역전형호박———소호위대상,우수화생소불동시단채집침적물화수체양품,분석침적물수해매화양화환원매활성수시간적변화。결과표명,소호수화형성기위5월15일-6월20일,지속기위6월20일-9월5일,지후진입수화소망기。제부분시단적감성린산매외,수화구침적물적뇨매、과양화경매、다분양화매활성균고우비수화구,수화구배경인자변화대도치매활성적영향인소다양화,이비수화구배경인자변화상대교소,2충수해매활성주요수침적물pH치화저물함량영향,이2충양화환원매활성수침적물TOC함량영향현저。종수화생소과정간,수화구뇨매、감성린산매활성변화명현대우비수화구,과양화경매화다분양화매야정현유사적변화추세。본연구표명,수화급기생소과정대침적물생물매적활성유영향,단영향대소여매적충류유관。
Enzymes play important roles in the biogeochemical cycles of mineral elements .However , their activities are apt to be impacted due to their sensitivity to environmental changes .Many studies concern the effects of eutrophication on enzyme activities in sediment , yet less attention is paid to the effects of the outbreak and perishing of water bloom .In this study , water and sediment samples were taken during the processes of outbreak and perishing of water bloom in a typical lake in the Yangtze River basin ( i.e.the Chaohu Lake ) , and the changes of hydrolase and oxidoreductase activ-ities in sediment in these processes were analyzed .Results showed that the water bloom formed between May 15 and June 20, sustained from June 20 to September 5, and then began to perish.Except for the alkaline phosphatase in some periods , activities of the urease , catalase and polyphenol oxidase in the sediment in the water bloom region were higher than those in the non-bloom region .In the water bloom region , environmental factors varied significantly with the out-break and perishing of water bloom , and many factors would cause the change of enzyme activity in sediments .In the non-bloom region , however , environmental factors were of less variation .The activity of two hydrolases were affected by the pH value and enzyme substrate in sediment , while the activity of two oxidoreductases were significantly affected by the TOC in sediment .Activities of the urease and alkaline phosphatase in the water bloom region showed greater varia -tion than those in the non-bloom region during the water bloom period .Activities of the catalase and polyphenol oxidase showed similar trends .Results showed that the enzyme activities in sediment were affected by the outbreak and perishing of water bloom .But the effects are dependent on enzyme species .