检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
22期
3373-3375
,共3页
杨悦%宋志伟%韩丽%马学华%李秋平%冯磊光
楊悅%宋誌偉%韓麗%馬學華%李鞦平%馮磊光
양열%송지위%한려%마학화%리추평%풍뢰광
精神分裂症%Reelin基因%DNA甲基化
精神分裂癥%Reelin基因%DNA甲基化
정신분렬증%Reelin기인%DNA갑기화
schizophrenia%Reelin gene%DNA methylation
目的:通过精神分裂症患者外周血中Reelin基因启动子区DNA甲基化状态的研究,探讨DNA甲基化与精神分裂症的关联。方法收集精神分裂症患者76例(患者组)和健康对照者60例(健康对照组),采用甲基化特异性PCR方法检测Reelin基因启动子区DNA甲基化状态。结果患者组甲基化阳性率为78.95%(60例),健康对照组甲基化阳性率为35%(21例),两组差异有统计学意义(χ2=4.971,P=0.026);Logistic回归分析显示, Reelin基因甲基化与精神分裂症有关(OR=13.084,95% CI:5.079~26.308)。结论 Reelin基因启动子区DNA甲基化与精神分裂症有关,精神分裂症患者外周血中Reelin基因启动子区DNA呈超甲基化状态,Reelin基因DNA甲基化是精神分裂症的危险因素。
目的:通過精神分裂癥患者外週血中Reelin基因啟動子區DNA甲基化狀態的研究,探討DNA甲基化與精神分裂癥的關聯。方法收集精神分裂癥患者76例(患者組)和健康對照者60例(健康對照組),採用甲基化特異性PCR方法檢測Reelin基因啟動子區DNA甲基化狀態。結果患者組甲基化暘性率為78.95%(60例),健康對照組甲基化暘性率為35%(21例),兩組差異有統計學意義(χ2=4.971,P=0.026);Logistic迴歸分析顯示, Reelin基因甲基化與精神分裂癥有關(OR=13.084,95% CI:5.079~26.308)。結論 Reelin基因啟動子區DNA甲基化與精神分裂癥有關,精神分裂癥患者外週血中Reelin基因啟動子區DNA呈超甲基化狀態,Reelin基因DNA甲基化是精神分裂癥的危險因素。
목적:통과정신분렬증환자외주혈중Reelin기인계동자구DNA갑기화상태적연구,탐토DNA갑기화여정신분렬증적관련。방법수집정신분렬증환자76례(환자조)화건강대조자60례(건강대조조),채용갑기화특이성PCR방법검측Reelin기인계동자구DNA갑기화상태。결과환자조갑기화양성솔위78.95%(60례),건강대조조갑기화양성솔위35%(21례),량조차이유통계학의의(χ2=4.971,P=0.026);Logistic회귀분석현시, Reelin기인갑기화여정신분렬증유관(OR=13.084,95% CI:5.079~26.308)。결론 Reelin기인계동자구DNA갑기화여정신분렬증유관,정신분렬증환자외주혈중Reelin기인계동자구DNA정초갑기화상태,Reelin기인DNA갑기화시정신분렬증적위험인소。
Objective To study the relationship between DNA methylation with schizophrenia by investigating the DNA methylation status of peripheral blood Reelin gene promoter in the patients with schizophrenia .Methods The Reelin gene promoter DNA methylation was measured in 76 patients with schizophrenia(patients group) and 60 healthy controls(healthy control group) by adopting methylation‐specific PCR .Results The methylation positive rate in the patients group was 78 .95% (60 cases) ,which in the healthy control group was 35% (21 cases) ,the differ‐ence was statistically significant(χ2 =4 .971 ,P=0 .026);the Logistic regression analysis showed that the Reelin gene methylation was correlated with schizophrenia(OR=13 .084 ,95% CI:5 .079-26 .308) .Conclusion The Reelin gene promoter DNA methylation is associated with schizophrenia ,and the peripheral blood Reelin gene promoter DNA methylation presents the supermethylation status in the patients with schizophrenia .Reelin gene DNA methylation is the risk factor for schizophrenia .