CAJ | 학술논문

目的：通过建立大鼠骨性关节炎（O A ）模型，研究大鼠血清和关节液中Ⅱ型前胶原羧基末端前肽（PⅡCP）水平变化，探讨体液PⅡCP对早期OA的诊断价值。方法将Wistar大鼠随机分成OA模型组和假手术组，用改良 Hulth法诱导大鼠膝关节OA模型。分别于造模后1～5周，每周每组抽取6只大鼠取关节液、血清及关节标本，用ELISA检测体液PⅡCP水平，关节软骨行大体观察评分和组织病理学评分。所得数据用方差分析比较各时间点两组间的差异性，比较PⅡCP水平和软骨评分随时间的变化规律，用Pearson相关系数和线性回归分析来检验体液PⅡCP水平的相关性。结果 OA模型组在术后第1周软骨组织学表现接近假手术组，从第2周开始逐渐出现明显的OA病理学改变，假手术组软骨组织学无明显改变。OA模型组体液PⅡCP水平从术后第1周开始较假手术组明显增高，并随时间推移差异性逐渐增大；假手术组体液PⅡCP始终维持在较低水平；血清与关节液PⅡCP水平具有良好相关性。结论大鼠OA模型中，血清和关节液PⅡCP水平在软骨出现明显病理变化之前就已经升高，PⅡCP水平与OA病理变化相符合，关节液和血清PⅡCP水平可作为OA早期诊断的参考指标。
목적：통과건립대서골성관절염（O A ）모형，연구대서혈청화관절액중Ⅱ형전효원최기말단전태（PⅡCP）수평변화，탐토체액PⅡCP대조기OA적진단개치。방법장Wistar대서수궤분성OA모형조화가수술조，용개량 Hulth법유도대서슬관절OA모형。분별우조모후1～5주，매주매조추취6지대서취관절액、혈청급관절표본，용ELISA검측체액PⅡCP수평，관절연골행대체관찰평분화조직병이학평분。소득수거용방차분석비교각시간점량조간적차이성，비교PⅡCP수평화연골평분수시간적변화규률，용Pearson상관계수화선성회귀분석래검험체액PⅡCP수평적상관성。결과 OA모형조재술후제1주연골조직학표현접근가수술조，종제2주개시축점출현명현적OA병이학개변，가수술조연골조직학무명현개변。OA모형조체액PⅡCP수평종술후제1주개시교가수술조명현증고，병수시간추이차이성축점증대；가수술조체액PⅡCP시종유지재교저수평；혈청여관절액PⅡCP수평구유량호상관성。결론대서OA모형중，혈청화관절액PⅡCP수평재연골출현명현병리변화지전취이경승고，PⅡCP수평여OA병리변화상부합，관절액화혈청PⅡCP수평가작위OA조기진단적삼고지표。
Objective To study the diagnostic value of C‐terminal propeptide of type Ⅱ procollagen(PⅡCP) in early osteoarthritis(OA) through monitoring the changes of PⅡCP in the synovial fluid and serum of the OA mod‐el rat .Methods Wistar rats were randomly divided into the OA model group and the sham operation group .The rat knee‐joint OA model was induced by the improved Hulth surgery method .The synovial fluid ,serum and joint speci‐mens were weekly taken from 6 rats in each group at 1-5 weeks after modeling ,the serum and synovial fluid PⅡCP levels were measured by ELISA .The general observation score and histopathological score of articular cartilage were obtained .The differences at various time points were compared between the two groups and the change rule of PⅡCP levels and cartilage scores with time was compared .The Pearson correlation coefficient and linear regression analysis were adopted to test the correlation between serum and synovial fluid PⅡCP levels .Results The histologic manifes‐tations of cartilage at postoperative 1 week in the OA model group were similar to those in the sham operation group , and the obvious pathologic change gradually started to appear at postoperative 2 weeks .The cartilage histology of the sham operation group had no obvious change .Compared with the sham operation group ,the serum and synovial fluid PⅡCP levels in the OA model group started to obviously increase at postoperative 1 week ,and the difference was gradually increased with time lapse;the humoral PⅡCP levels of the sham operation group maintained a lower level from beginning to end;the serum PⅡCP level had a good correlation with the synovial fluid PⅡCP level .Conclusion In rat OA model ,the serum and synovial fluid P Ⅱ CP levels are increased before the cartilage appears obvious pathological change ,and the P Ⅱ CP levels are consistent with the OA pathological change ,so the serum and synovial fluid P Ⅱ CP levels could be used as the reference indicators of OA early diagnosis .