CAJ | 학술논문

目的：通过酶联免疫吸附试验（ELISA ）检测人类免疫缺陷病毒（HIV ）抗体，旨在设置科学、合理的HIV抗体检测的“灰区”范围，减少 HIV抗体阳性的漏检率。方法采用ELISA对吸光度值／临界值（S／CO）介于大于0．8～＜1．2的30例临界值患者血清进行HIV抗体检测，以此S／CO值，设置“灰区”范围；对于S／CO介于灰区的标本进行免疫印迹确证。结果设置灰区参考范围为0．796～1．204；共有46例处于“灰区”的血清标本，经免疫印迹确证为阳性3例，不确定1例。结论 ELISA检测HIV抗体应设置合理的“灰区”范围，对灰区的标本进行确证，对减少HIV抗体阳性漏检具有重要的临床意义。
목적：통과매련면역흡부시험（ELISA ）검측인류면역결함병독（HIV ）항체，지재설치과학、합리적HIV항체검측적“회구”범위，감소 HIV항체양성적루검솔。방법채용ELISA대흡광도치／림계치（S／CO）개우대우0．8～＜1．2적30례림계치환자혈청진행HIV항체검측，이차S／CO치，설치“회구”범위；대우S／CO개우회구적표본진행면역인적학증。결과설치회구삼고범위위0．796～1．204；공유46례처우“회구”적혈청표본，경면역인적학증위양성3례，불학정1례。결론 ELISA검측HIV항체응설치합리적“회구”범위，대회구적표본진행학증，대감소HIV항체양성루검구유중요적림상의의。
Objective To set the scientific and rational gray area range of HIV antibody detection by ELISA for reducing the missed detection rate of HIV antibody detection .Methods Thirty serum specimens with the critical value range of 0 .8<S/CO<1 .2 were performed the HIV antibody detection ,the gray area range was set with this S/CO value;for the samples with S/CO between the gray area ,the Western blot analysis was conducted for verification . Results The reference range of gray area was set at 0 .796-1 .204 .46 serum samples in gray area were verified via Western blot analysis ,in which 3 specimens were positive and 1 specimen was indeterminacy .Conclusion The ration‐al gray area range should be set for ELISA assay in detecting HIV antibody ,conducting the verification of the gray area samples has an important clinical significance for reducing the missed detection of HIV antibody .