CAJ | 학술논문

目的：探讨不同的血样采集管，不同存放时间及不同存放温度对同型半胱氨酸（Hcy ）测定结果的影响。方法选取32例健康体检者，分别用普通真空采血管、促凝真空采血管、肝素锂抗凝真空采血管、EDTA‐K2抗凝真空采血管各抽取两管静脉血，将同一受检者的相同采血管分别分入室温组和冷藏组，编号并于室温放置0．5h离心，离心后立即将冷藏组置于冰箱，并检测室温组H cy浓度；同时立刻吸取每例受检者普通真空采血管及肝素锂抗凝真空采血管血清及血浆200μL于1 mL EP管并置于2～8℃冰箱冷藏保存，为分离组。在离心后3、6、9 h测定室温组及冷藏组Hcy浓度，测定完成后选取冷藏组普通真空采血管及肝素锂抗凝真空采血管继续在冰箱中放置为未分离组，并在24、48 h测定分离组与未分离组H cy浓度。结果4种采血管标本的 H cy即刻值血清标本高于血浆标本（P＜0．05）。同组相同采血管放置3、6、9 h与各自即刻值比较差异无统计学意义（P＞0．05）。在相同测定时间比较同种采血管室温组与冷藏组的Hcy浓度结果，9 h测定值比较差异有统计学意义（P＜0．05）。不及时分离血细胞层，普通真空采血管血清和肝素锂抗凝真空采血管血浆中 Hcy浓度在2～8℃下，48 h结果与即刻值比较差异均有统计学意义（P＜0．05）。结论不同的血样采集管，不同存放时间及不同存放温度都会影响循环酶法H cy的测定结果。
목적：탐토불동적혈양채집관，불동존방시간급불동존방온도대동형반광안산（Hcy ）측정결과적영향。방법선취32례건강체검자，분별용보통진공채혈관、촉응진공채혈관、간소리항응진공채혈관、EDTA‐K2항응진공채혈관각추취량관정맥혈，장동일수검자적상동채혈관분별분입실온조화랭장조，편호병우실온방치0．5h리심，리심후립즉장랭장조치우빙상，병검측실온조H cy농도；동시립각흡취매례수검자보통진공채혈관급간소리항응진공채혈관혈청급혈장200μL우1 mL EP관병치우2～8℃빙상랭장보존，위분리조。재리심후3、6、9 h측정실온조급랭장조Hcy농도，측정완성후선취랭장조보통진공채혈관급간소리항응진공채혈관계속재빙상중방치위미분리조，병재24、48 h측정분리조여미분리조H cy농도。결과4충채혈관표본적 H cy즉각치혈청표본고우혈장표본（P＜0．05）。동조상동채혈관방치3、6、9 h여각자즉각치비교차이무통계학의의（P＞0．05）。재상동측정시간비교동충채혈관실온조여랭장조적Hcy농도결과，9 h측정치비교차이유통계학의의（P＜0．05）。불급시분리혈세포층，보통진공채혈관혈청화간소리항응진공채혈관혈장중 Hcy농도재2～8℃하，48 h결과여즉각치비교차이균유통계학의의（P＜0．05）。결론불동적혈양채집관，불동존방시간급불동존방온도도회영향순배매법H cy적측정결과。
Objective To study the influence of different blood sample collection tubes ,different storage time and different storage temperatures on the results of homocysteine(Hcy) detection by enzymatic cycle assay .Methods The venous blood sample was collected from 32 individuals undergoing the healthy physical examination .The gen‐eral vacuum blood collection tubes ,procoagulant vacuum blood collection tubes ,lithium heparin anticoagulation vacu‐um blood collection tubes and EDTA‐K2 anticoagulation vacuum blood collection tubes were respectively adopted to collect 2 tubes of venous blood for each kind of tube .The same blood tubes from the same person were divided into the room temperature group and the cold storage group respectively ,centrifuged after giving serial number and sto‐ring for 0 .5 h at the room temperature ,Hcy in the room temperature group was detected at once ,while the cold stor‐age group was stored in the fridge;meanwhile 200 μL of serum and plasma were extracted from the general tube and lithium heparin anticoagulation tube and placed into 1 mL EP tube for storage in fridge at 2 -8 ℃ (separation group) .The Hcy levels in the room temperature group and the cold storage group were detected at 3 ,6 ,9 h after cen‐trifugation .After detection ,the general tube and lithium heparin anticoagulation tube in the cold storage group were continuously placed in fridge(non‐separation group) .The Hcy levels in the separation group and the non‐separation group were detected at 24 ,48 h .Results The serum Hcy instant values of 4 kinds of blood collection tube for serum specimen were significantly higher than those for plasma specimen(P<0 .05) .The Hcy levels had no statistically sig‐nificant differences between the placing for 3 ,6 ,9 h by the same collection tube in the same group and the instant val‐ues(P>0 .05) .In the comparison of the Hcy detection results of the same blood collection tube at the same detection time between the room temperature group and the cold storage group ,the detection value at 9 h had statistical differ‐ence(P<0 .05) .The Hcy levels at 48 h under 2-8 ℃ in the general tube serum and lithium heparin anticoagulation tube plasma without timely separating blood cell layers had statistical difference compared with the results detected at 48 h(P<0 .05) .Conclusion Different blood collection tubes ,different storage time and different storage tempera‐tures will affect the detection results of Hcy by enzymatic cycle assay .