海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
21期
3086-3088
,共3页
乙二胺四乙酸%枸橼酸钠%肝素锂%假性血小板减少症%血小板计数
乙二胺四乙痠%枸櫞痠鈉%肝素鋰%假性血小闆減少癥%血小闆計數
을이알사을산%구연산납%간소리%가성혈소판감소증%혈소판계수
Ethylene diamine tetraacetic acid (EDTA)%Sodium citrate%Heparin lithium%Pseudothrombocyto-penia%Platelet count
目的 探讨乙二胺四乙酸依赖性假性血小板减少症(EDTA-PTCP)几种检测方法的可靠性.方法 对5例EDTA-PTCP患者和20位健康体检者同时用经乙二胺四乙酸二钾(EDTA-K2)、枸橼酸钠和肝素锂三种抗凝管及末梢血稀释的方式采集标本,分别用血液分析仪在三种抗凝管采集标本后5 min、30 min、60 min测定血小板计数,采集末梢血用显微镜手工计数血小板,对所有标本涂片染色观察血小板形态.结果 5例EDTA-PTCP患者EDTA-K2采血管采血后30 min和60 min血小板计数(PLT)比5 min时测定结果显著偏低,差异具有统计学意义(P<0.05).DTA-PTCP患者EDTA-K2采血后30 min和60 min抗凝血涂片染色均见明显的血小板聚集现象,而健康体检者EDTA-K2抗凝血涂片及未抗凝血直接涂片染色见血小板散在分布.在采血30 min后测定,EDTA-K2采血管PLT明显低于枸橼酸钠、肝素锂及手工计数结果,差异均具有统计学意义(P<0.05);20例健康体检者在采血后5 min、30 min、60 min时EDTA-K2采血管PLT与枸橼酸钠、肝素锂、手工计数的结果比较差异均无统计学意义(P>0.05).结论 对疑似EDTA-PTCP患者可以用EDTA-K2采血管重新采血立即测定,或不使用抗凝剂直接抽血立即测定,也可以使用枸橼酸钠或肝素锂抗凝管初步确认,同时涂片染色并手工计数得到正确的结果.
目的 探討乙二胺四乙痠依賴性假性血小闆減少癥(EDTA-PTCP)幾種檢測方法的可靠性.方法 對5例EDTA-PTCP患者和20位健康體檢者同時用經乙二胺四乙痠二鉀(EDTA-K2)、枸櫞痠鈉和肝素鋰三種抗凝管及末梢血稀釋的方式採集標本,分彆用血液分析儀在三種抗凝管採集標本後5 min、30 min、60 min測定血小闆計數,採集末梢血用顯微鏡手工計數血小闆,對所有標本塗片染色觀察血小闆形態.結果 5例EDTA-PTCP患者EDTA-K2採血管採血後30 min和60 min血小闆計數(PLT)比5 min時測定結果顯著偏低,差異具有統計學意義(P<0.05).DTA-PTCP患者EDTA-K2採血後30 min和60 min抗凝血塗片染色均見明顯的血小闆聚集現象,而健康體檢者EDTA-K2抗凝血塗片及未抗凝血直接塗片染色見血小闆散在分佈.在採血30 min後測定,EDTA-K2採血管PLT明顯低于枸櫞痠鈉、肝素鋰及手工計數結果,差異均具有統計學意義(P<0.05);20例健康體檢者在採血後5 min、30 min、60 min時EDTA-K2採血管PLT與枸櫞痠鈉、肝素鋰、手工計數的結果比較差異均無統計學意義(P>0.05).結論 對疑似EDTA-PTCP患者可以用EDTA-K2採血管重新採血立即測定,或不使用抗凝劑直接抽血立即測定,也可以使用枸櫞痠鈉或肝素鋰抗凝管初步確認,同時塗片染色併手工計數得到正確的結果.
목적 탐토을이알사을산의뢰성가성혈소판감소증(EDTA-PTCP)궤충검측방법적가고성.방법 대5례EDTA-PTCP환자화20위건강체검자동시용경을이알사을산이갑(EDTA-K2)、구연산납화간소리삼충항응관급말소혈희석적방식채집표본,분별용혈액분석의재삼충항응관채집표본후5 min、30 min、60 min측정혈소판계수,채집말소혈용현미경수공계수혈소판,대소유표본도편염색관찰혈소판형태.결과 5례EDTA-PTCP환자EDTA-K2채혈관채혈후30 min화60 min혈소판계수(PLT)비5 min시측정결과현저편저,차이구유통계학의의(P<0.05).DTA-PTCP환자EDTA-K2채혈후30 min화60 min항응혈도편염색균견명현적혈소판취집현상,이건강체검자EDTA-K2항응혈도편급미항응혈직접도편염색견혈소판산재분포.재채혈30 min후측정,EDTA-K2채혈관PLT명현저우구연산납、간소리급수공계수결과,차이균구유통계학의의(P<0.05);20례건강체검자재채혈후5 min、30 min、60 min시EDTA-K2채혈관PLT여구연산납、간소리、수공계수적결과비교차이균무통계학의의(P>0.05).결론 대의사EDTA-PTCP환자가이용EDTA-K2채혈관중신채혈립즉측정,혹불사용항응제직접추혈립즉측정,야가이사용구연산납혹간소리항응관초보학인,동시도편염색병수공계수득도정학적결과.
Objective To investigate the reliability of the methods for detecting EDTA-dependent pseudo-thrombocytopenia (EDTA-PTCP). Methods Blood samples were collected from 5 patients of EDTA-PTCP and 20 healthy subjects by three anticoagulant tubes (EDTA-K2, sodium citrate and heparin lithium) and peripheral blood dilu-tion. Platelets were determined by haematology analyzer 5 min, 30 min, 60 min after the samples were collected by an-ticoagulant tubes, as well as determined manually under the microscope for peripheral blood samples collected. Smear staining was performed to observe platelet morphology. Results In the five DTA-PTCP patients, the platelet count of EDTA-K2 anticoagulant tube was significantly lower at 30 min and 60 min after sample collection than at 5 min (P<0.05). Platelet of EDTA-K2 anticoagulant tube from the 5 patients showed significant aggregation during smear staining, while the platelet of EDTA-K2 anticoagulant tube from the 20 healthy subjects and the peripheral blood (man-ual counting) showed scattered distribution. 30 min after blood collection, the platelet count of EDTA-K2 anticoagu-lant tube was significantly lower than that of sodium citrate anticoagulant tube, heparin lithium anticoagulant tube, manual counting (P<0.05). In 20 healthy subjects, no significant difference exist in the results between EDTA-K2 anti-coagulant tube and sodium citrate anticoagulant tube, heparin lithium anticoagulant tube, manual counting (P>0.05). Conclusion For suspected patients of EDTA-PTCP, blood samples can be collected by EDTA-K2 anticoagulant tube or draw directly without anticoagulant for immediate platelet determination. Samples can also be collected by using so-dium citrate or lithium heparin anticoagulant tube for initial identification. Smear staining and manual counting should be performed to obtain correct results.
