广州化工
廣州化工
엄주화공
Guangzhou Chemical Industry
2015年
21期
116-118
,共3页
双氯芬酸钾%干混悬剂%含量测定%高效液相色谱法
雙氯芬痠鉀%榦混懸劑%含量測定%高效液相色譜法
쌍록분산갑%간혼현제%함량측정%고효액상색보법
diclofenac potassium%dry suspension agent%determination%high performance liquid chromatography
建立了高效液相色谱法测定双氯芬酸钾干混悬剂含量的测定方法。色谱柱为 Kromasil C18(250 mm ×4.6 mm,2.5μm),流动相：甲醇-水-冰醋酸(80：20：0.8, V：V：V),检测波长为276 nm,流速为1.0 mL·min-1,进样量为20μL。结果表明： y＝40999x+119667(r＝0.9995, n＝7)线性范围为4.0~6.0μg·mL-1；最低检测限为1.66 ng,定量限为5.56 ng；平均回收率为99.57%。该方法简便、准确、适用于双氯芬酸钾干混悬剂的含量测定。
建立瞭高效液相色譜法測定雙氯芬痠鉀榦混懸劑含量的測定方法。色譜柱為 Kromasil C18(250 mm ×4.6 mm,2.5μm),流動相：甲醇-水-冰醋痠(80：20：0.8, V：V：V),檢測波長為276 nm,流速為1.0 mL·min-1,進樣量為20μL。結果錶明： y＝40999x+119667(r＝0.9995, n＝7)線性範圍為4.0~6.0μg·mL-1；最低檢測限為1.66 ng,定量限為5.56 ng；平均迴收率為99.57%。該方法簡便、準確、適用于雙氯芬痠鉀榦混懸劑的含量測定。
건립료고효액상색보법측정쌍록분산갑간혼현제함량적측정방법。색보주위 Kromasil C18(250 mm ×4.6 mm,2.5μm),류동상：갑순-수-빙작산(80：20：0.8, V：V：V),검측파장위276 nm,류속위1.0 mL·min-1,진양량위20μL。결과표명： y＝40999x+119667(r＝0.9995, n＝7)선성범위위4.0~6.0μg·mL-1；최저검측한위1.66 ng,정량한위5.56 ng；평균회수솔위99.57%。해방법간편、준학、괄용우쌍록분산갑간혼현제적함량측정。
The high performance liquid chromatography ( HPLC) method for the content of diclofenac potassium dry suspension agent was established to study the high performance liquid chromatography ( HPLC) method for the content of diclofenac potassium dry suspension agent, using chromatographic column for Kromasil C18 ( 250 mm × 4. 6 mm, 2. 5 μm), mobile phase methanol-water-acetic acid glacial (80:20:0. 8), detection wavelength of 276 nm, flow rate of 1. 0 mL·min-1, sample amount to 20 μL. Results showed that 40999x+y=40999 (r=0. 9995, n=7) with linear range of 4. 0~6. 0 μg·mL-1, the minimum detection limit was 1. 66 ng, quantitative limit was 5. 56 ng. The average recovery was 99. 57%. The method is suitable for determination of the diclofenac potassium dry suspension agent, and it is convenient, accurate, simple and feasible.
