CAJ | 학술논문

目的探讨左卡尼汀(LCN)对犬心肌挫伤后心脏功能的治疗作用. 方法选择雄性健康杂种犬24只,按随机数字表法分为挫伤组、低剂量LCN治疗组(LCN1组)和高剂量LCN治疗组(LCN2组),每组8只;均建立心肌挫伤动物模型.LCN1组、LCN2组在模型建立前连续3d及模型建立后即刻分别使用LCN 100 mg/kg、200 mg/kg.各组于伤前30 min、伤后30 min、1,2,4,6h测定左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室内压最大上升及最大下降速率(±dp/dtmax);上述时相点抽取静脉血,ELISA法测定肌酸激酶同工酶(CK-MB)和肌钙蛋白I(cTnI)水平,硫代巴比妥酸法测定丙二醛(MDA)含量;伤前1d和伤后5h行超声心动图检查室壁运动积分指数(WMSI);伤后6h处死动物,检测心肌组织三磷酸腺苷(ATP)含量. 结果三组在模型建立后LVSP、±dp/dtmax出现下降趋势,LVEDP逐渐增高,LCN1、LCN2组变化程度较小.三组血中CK-MB和cTnI水平在伤后上升,而LCN1组、LCN2组低于同时相点挫伤组水平,以LCN2组最显著.三组MDA表达量逐渐增高,挫伤组增高幅度较LCN1组、LCN2组显著.三组伤后5hWMSI均上升,挫伤组(2.46±0.31)高于LCN1组(1.91±0.23)和LCN2组(1.85±0.25)(P＜0.05),但LCN1组与LCN2组比较差异无统计学意义(P＞0.05).挫伤组ATP含量为(11.3±1.6) μg/g,低于LCN1组的(15.7 ±2.8) μg/g和LCN2组的(21.3±3.9) μg/g (P ＜0.05),LCN1组ATP含量低于LCN2组(P＜0.05). 结论 LCN可以减轻犬心肌挫伤后心脏功能的受损程度,可能与LCN可以改善挫伤心肌能量失衡有关.
목적 탐토좌잡니정(LCN)대견심기좌상후심장공능적치료작용. 방법 선택웅성건강잡충견24지,안수궤수자표법분위좌상조、저제량LCN치료조(LCN1조)화고제량LCN치료조(LCN2조),매조8지;균건립심기좌상동물모형.LCN1조、LCN2조재모형건립전련속3d급모형건립후즉각분별사용LCN 100 mg/kg、200 mg/kg.각조우상전30 min、상후30 min、1,2,4,6h측정좌실수축압(LVSP)、좌실서장말압(LVEDP)、좌실내압최대상승급최대하강속솔(±dp/dtmax);상술시상점추취정맥혈,ELISA법측정기산격매동공매(CK-MB)화기개단백I(cTnI)수평,류대파비타산법측정병이철(MDA)함량;상전1d화상후5h행초성심동도검사실벽운동적분지수(WMSI);상후6h처사동물,검측심기조직삼린산선감(ATP)함량. 결과 삼조재모형건립후LVSP、±dp/dtmax출현하강추세,LVEDP축점증고,LCN1、LCN2조변화정도교소.삼조혈중CK-MB화cTnI수평재상후상승,이LCN1조、LCN2조저우동시상점좌상조수평,이LCN2조최현저.삼조MDA표체량축점증고,좌상조증고폭도교LCN1조、LCN2조현저.삼조상후5hWMSI균상승,좌상조(2.46±0.31)고우LCN1조(1.91±0.23)화LCN2조(1.85±0.25)(P＜0.05),단LCN1조여LCN2조비교차이무통계학의의(P＞0.05).좌상조ATP함량위(11.3±1.6) μg/g,저우LCN1조적(15.7 ±2.8) μg/g화LCN2조적(21.3±3.9) μg/g (P ＜0.05),LCN1조ATP함량저우LCN2조(P＜0.05). 결론 LCN가이감경견심기좌상후심장공능적수손정도,가능여LCN가이개선좌상심기능량실형유관.
Objective To investigate the effect of L-carnitine (LCN) on heart function following myocardial contusion in mongrel dogs.Methods Twenty-four male mongrel dogs were randomly divided into myocardial contusion group,low dose LCN group (LCN1 group),and high dose LCN group (LCN2 group) with 8 dogs per group,according to the random number table.The model of myocardial contusion was established in all groups.LCN1 and LCN2 groups were respectively treated with 100 mg/kg and 200 mg/kg LCN for three consecutive days before the injury and afterward.Left ventricular systolic pressure (LVSP),left ventricular end diastolic pressure (LVEDP),left ventricular ± dp/dt were recorded 30 min before injury,and 30 min,1 h,2 h,4 h as well as 6 h after injury.Blood samples were withdrawn from femoral vein to detect the levels of creatine kinase isozyme (CK-MB),cardiac troponin I (cTnI),and malondialdehyde (MDA).Wall motion score index (WMSI) was observed using the ultrasound examination 1 day before and 5 hours after injury.Myocardial tissue was available at 6 hours after injury for the examination of content of adenosine triphosphate (ATP).Results LVSP and ± dp/dtmax appeared a downward trend after injury,while a gradual increase in LVEDP.And the changes were relatively smaller with LCN intervention,especially in LCN2 group.Levels of CK-MB,cTnI and MDA increased after injury,but the same time point showed lower levels in LCN groups (especially in LCN2 group) as compared to myocardial contusion group (P ＜ 0.05).WMSI increased 5 hours after the injury,but the increase was higher in myocardial contusion group (2.46 ±0.31) than that in LCN1 group (1.91 ± 0.23) and LCN2 group (1.85 ± 0.25) (P ＜ 0.05).There was no significant difference in WMSI between the two LCN groups (P ＞ 0.05).Level of ATP in myocardial contusion group was (11.3 ± 1.6) μg/g,which was improved after LCN intervention (P ＜ 0.05),and the improvement was much higherin LCN2 group than in LCN1 group[(21.3±3.9) ug/g vs (15.7±2.8)ug/g] (P＜0.05).Conclusion LCN can alleviate the adverse effect of myocardial contusion on cardiac function in mongrel dogs,which may be associated with the reduced energy imbalance induced by LCN.