CAJ | 학술논문

目的探讨盐酸戊乙奎醚(PHC)对大鼠内毒素(LPS)性脑损伤后血脑屏障(BBB)通透性的影响,为临床治疗提供参考. 方法选择清洁级成年雄性SD大鼠81只,按随机数字表法分为对照组、LPS性脑损伤组(LPS组)和PHC治疗组(PHC组),每组27只.LPS组和PHC组均制备内毒素脑损伤模型,PHC组按2 mg/kg腹腔注射PHC,LPS组和对照组腹腔注射与PHC组等量的等渗盐水.荧光显微镜观察各组脑组织伊文思蓝渗出,分光光度计测定各组脑组织中伊文思蓝含量,ELISA法测定各组肿瘤坏死因子-α(TNF-α)含量,通过检测脑组织干/湿重计算脑组织含水量. 结果 LPS组有大量红色荧光物质,PHC组红色荧光物质明显减少.对照组左侧脑皮质伊文思蓝含量[(10.2±1.4) μg/g]、海马伊文思蓝含量[(11.3 ±0.8)μg/g]和脑组织含水量[(73.5±1.8)％]均显著低于LPS组[(234.8 ±14.7)μg/g、(23.1±1.9)μg/g、(85.3±1.6)％]和PHC组[(71.0±8.7) μg/g、(15.2±1.9)μg/g、(78.9±1.2)％](P＜0.01);PHC组均低于LPS组(P＜0.01).对照组血清、脑脊液、脑组织中TNF-α含量[(40.6±4.4) pg/ml、(84.0±8.6)pg/ml、(156.3±11.8)pg/ml]均显著低于LPS组[(93.4±7.2)pg/ml、(176.4±11.6)pg/ml、(280.8±19.4) pg/ml]和PHC组[(79.9 ± 5.9) pg/ml、(146.2±7.4) pg/ml、(216.9±14.5)pg/ml] (P＜0.01);PHC组均低于LPS组(P＜0.01). 结论 PHC对LPS性脑损伤大鼠BBB具有保护作用.
목적 탐토염산무을규미(PHC)대대서내독소(LPS)성뇌손상후혈뇌병장(BBB)통투성적영향,위림상치료제공삼고. 방법 선택청길급성년웅성SD대서81지,안수궤수자표법분위대조조、LPS성뇌손상조(LPS조)화PHC치료조(PHC조),매조27지.LPS조화PHC조균제비내독소뇌손상모형,PHC조안2 mg/kg복강주사PHC,LPS조화대조조복강주사여PHC조등량적등삼염수.형광현미경관찰각조뇌조직이문사람삼출,분광광도계측정각조뇌조직중이문사람함량,ELISA법측정각조종류배사인자-α(TNF-α)함량,통과검측뇌조직간/습중계산뇌조직함수량. 결과 LPS조유대량홍색형광물질,PHC조홍색형광물질명현감소.대조조좌측뇌피질이문사람함량[(10.2±1.4) μg/g]、해마이문사람함량[(11.3 ±0.8)μg/g]화뇌조직함수량[(73.5±1.8)％]균현저저우LPS조[(234.8 ±14.7)μg/g、(23.1±1.9)μg/g、(85.3±1.6)％]화PHC조[(71.0±8.7) μg/g、(15.2±1.9)μg/g、(78.9±1.2)％](P＜0.01);PHC조균저우LPS조(P＜0.01).대조조혈청、뇌척액、뇌조직중TNF-α함량[(40.6±4.4) pg/ml、(84.0±8.6)pg/ml、(156.3±11.8)pg/ml]균현저저우LPS조[(93.4±7.2)pg/ml、(176.4±11.6)pg/ml、(280.8±19.4) pg/ml]화PHC조[(79.9 ± 5.9) pg/ml、(146.2±7.4) pg/ml、(216.9±14.5)pg/ml] (P＜0.01);PHC조균저우LPS조(P＜0.01). 결론 PHC대LPS성뇌손상대서BBB구유보호작용.
Objective To detect the effect of penhyclidine hydrochloride (PHC) on blood-brain barrier permeability in rats with lipopolysaccharide (LPS)-induced brain injury.Methods Eighty-one clear adult male SD rats were divided into control group,LPS group and PHC group according to the random number table,with 27 rats per group.The model of LPS-induced brain injury was established both in LPS and PHC groups.Rats were treated intraperitoneally with 2 mg/kg PHC in PHC group,while with the same volume of normal saline in control and LPS groups.Evans blue propagation through the brain was detected under the fluorescence microscope.Evans blue content was measured with a spectrophotometer.TNF-α content was measured with the Elisa assay.Brain water content was assessed with wet-to-dry weight ratios.Results Evans blue distribution in the cortex was obvious in LPS group,but decreased in PHC group.In control group contents of Evans blue in the cortex [(10.2 ± 1.4) μg/g] and in the hippocampus[(11.3 ±0.8)μg/g] lowered as compared to these in LPS group [(234.8 ± 14.7) and (23.1 ± 1.9)μg/g] and PHC group [(71.0 ±8.7) and (15.2 ± 1.9)μg/g] (P＜0.01).Brain water content in control group was (73.5 ± 1.8) ％,increased to (85.3 ± 1.6) ％ in LPS group and (78.9 ± 1.2)％ in PHC group (P ＜ 0.01).In control group contents of TNF-α in blood serum [(40.6 ± 4.4) pg/ml],cerebrospinal fluid [(84.0 ± 8.6) pg/ml] and brain tissue [(156.3 ± 11.8) pg/ml] were significantly lower than these in LPS group [(93.4 ± 7.2),(176.4 ± 11.6) and (280.8 ± 19.4)pg/ml] and PHCgroup[(79.9 ±5.9),(146.2±7.4) and (216.9±14.5) pg/ml] (P＜ 0.01).And in comparison,all the measurements were lower in PHC group than in LPS group (P ＜ 0.01).Conclusion PHC can protect the BBB in rats with LPS-induced brain injury.