CAJ | 학술논문

目的：探究脊髓损伤后大鼠学习记忆功能与海马病理变化特点及关系。方法36只成年雌性Sprague-Dawley大鼠随机分为假手术组(n=18)和损伤组(n=18)。用Allen法打击T10脊髓制备脊髓损伤模型，打击强度为10 g×25 mm。损伤后1 d、3 d及1~5周每周1次行BBB后肢功能评分；术后5周时检测运动诱发电位，行Morris水迷宫测试；术后1周、3周、5周各组分别取4只大鼠行HE染色，检测海马细胞形态变化。结果术后每个时间点，损伤组BBB评分均低于假手术组(P<0.05)。术后5周，损伤组运动诱发电位N1、P1波潜伏期显著长于假手术组(P<0.001)，振幅显著低于假手术组(P<0.001)。Morris水迷宫测试，损伤组到达平台潜伏期显著长于假手术组(P<0.001)，在目标象限的探索时间显著少于假手术组(P<0.001)，且多以系统定位或环形定位方法寻找目标平台，而假手术组则以空间定位方法为主。HE染色显示，1周时，损伤组海马组织有少量细胞形态异常；随着时间延长，海马内形态异常细胞逐渐增多，存活细胞逐渐减少；假手术组HE染色基本正常。结论脊髓损伤可引起大鼠学习记忆功能障碍，可能与海马细胞损伤有关。
목적：탐구척수손상후대서학습기억공능여해마병리변화특점급관계。방법36지성년자성Sprague-Dawley대서수궤분위가수술조(n=18)화손상조(n=18)。용Allen법타격T10척수제비척수손상모형，타격강도위10 g×25 mm。손상후1 d、3 d급1~5주매주1차행BBB후지공능평분；술후5주시검측운동유발전위，행Morris수미궁측시；술후1주、3주、5주각조분별취4지대서행HE염색，검측해마세포형태변화。결과술후매개시간점，손상조BBB평분균저우가수술조(P<0.05)。술후5주，손상조운동유발전위N1、P1파잠복기현저장우가수술조(P<0.001)，진폭현저저우가수술조(P<0.001)。Morris수미궁측시，손상조도체평태잠복기현저장우가수술조(P<0.001)，재목표상한적탐색시간현저소우가수술조(P<0.001)，차다이계통정위혹배형정위방법심조목표평태，이가수술조칙이공간정위방법위주。HE염색현시，1주시，손상조해마조직유소량세포형태이상；수착시간연장，해마내형태이상세포축점증다，존활세포축점감소；가수술조HE염색기본정상。결론척수손상가인기대서학습기억공능장애，가능여해마세포손상유관。
Objective To explore the learning and memory impairment and pathology in hippocampus in rats after spinal cord contu-sion. Methods 36 adult female Sprague-Dawley rats were randomly divided into sham group (n=18) and spinal cord injury group (n=18). Spinal cord injury model at T10 was established with modified Allen's technique (10 g × 25 mm). The hindlimbs behavior of rats was rated with Basso-Beattie-Bresnahan (BBB) scores once a week for 5 weeks. They were tested with motor evoked potentials (MEP) and Morris wa-ter maze 5 weeks after injury. The pathology of hippocampus was detected with HE staining 1 week, 3 weeks and 5 weeks after injury, 4 rats in a group, repectively. Results The BBB scores were significantly lower in the spinal cord injury group than in the sham group at each time point (P<0.05). The latencies of both N1 and P1 wave of MEP were significantly longer in the spinal cord injury group than in the sham group (P<0.001), while the amplitudes were significantly less (P<0.001). For the Morris water maze, the latency of arrival platform were sig-nificantly longer in the spinal cord injury group than in the sham group (P<0.001), and the time in target was significantly less (P<0.001), with more systematic positioning or annular positioning, while the sham group with more space-based positioning. Morphologically abnor-mal cells in hippocampus gradually increased since the first week after injury, with the decrease of cells survival, while it was normal in the sham group. Conclusion Spinal cord contusion can cause learning and memory impairment in rats, which may be related to injury in hippo-campus.