CAJ | 학술논문

目的：观察养血清脑颗粒对血管性痴呆大鼠海马CA1区超微结构及p38丝裂原活化蛋白激酶(p38MAPK)蛋白表达的影响。方法180只Sprague-Dawley大鼠随机分为假手术组、血管性痴呆模型组(模型组)和养血清脑颗粒治疗组(治疗组)，采用改良的Pulsinelli四血管阻断法制作血管性痴呆模型，分别在术后1、2、4、8周采用透射电镜观察大鼠海马CA1区超微结构变化，免疫组化法和Western blotting法检测海马CA1区p38MAPK水平。结果模型组海马CA1区神经元大量固缩，细胞核溶解，异染色质边集，线粒体肿胀。治疗组海马CA1区神经元核膜较完整，核染色质分布较均匀，胞质内线粒体及其他细胞器基本正常。与假手术组相比，模型组各时间点p38MAPK蛋白表达明显增多(P<0.01)，4周时达高峰；与模型组比较，治疗组各时间点p38MAPK蛋白表达明显减少(P<0.01)。结论养血清脑颗粒可改善血管性痴呆模型大鼠海马CA1区损伤的神经元超微结构，可能与抑制p38MAPK蛋白的表达有关。
목적：관찰양혈청뇌과립대혈관성치태대서해마CA1구초미결구급p38사렬원활화단백격매(p38MAPK)단백표체적영향。방법180지Sprague-Dawley대서수궤분위가수술조、혈관성치태모형조(모형조)화양혈청뇌과립치료조(치료조)，채용개량적Pulsinelli사혈관조단법제작혈관성치태모형，분별재술후1、2、4、8주채용투사전경관찰대서해마CA1구초미결구변화，면역조화법화Western blotting법검측해마CA1구p38MAPK수평。결과모형조해마CA1구신경원대량고축，세포핵용해，이염색질변집，선립체종창。치료조해마CA1구신경원핵막교완정，핵염색질분포교균균，포질내선립체급기타세포기기본정상。여가수술조상비，모형조각시간점p38MAPK단백표체명현증다(P<0.01)，4주시체고봉；여모형조비교，치료조각시간점p38MAPK단백표체명현감소(P<0.01)。결론양혈청뇌과립가개선혈관성치태모형대서해마CA1구손상적신경원초미결구，가능여억제p38MAPK단백적표체유관。
Objective To observe the effect of Yangxue Qingnao Granule on the ultrastructures and expression of p38 mitogen activated protein kinases (p38MAPK) in CA1 area of hippocampus in vascular dementia rats. Methods 180 Sprague-Dawley rats were randomly di-vided into sham group, vascular dementia model group (model group) and Yangxue Qingnao Granule treatment group (treatment group). The vascular dementia was modeled with modified Pulsineli's four-vessel occlusion. The ultrastructure of CA1 area was observed with trans-mission electron microscope, while the expression of p38MAPK in CA1 area was detected with immunohistochemstry and Western blotting 1, 2, 4 and 8 weeks after modeling. Results In the model group, pyknosis, nuclear dissolution, heterochromatin margination and mitochon-dria swelling were found in most of the neurons in CA1. In the treatment group, the distribution of chromatin was well-proportioned, and mi-tochondrion and other organelle were normal. In the model group, the expression of p38MAPK increased at each time point compared with the sham group (P<0.01), and peaked 4 weeks after modeling, and decreased in the treatment group compared with the model group (P<0.01). Conclusion Yangxue Qingnao Granule can improve the ultrastructure of neuronal in CA1 area of hippocampus of vascular dementia rats, which may relate with the inhibition of the expression of p38MAPK.