亚太热带生物医学杂志（英文版）
亞太熱帶生物醫學雜誌（英文版）
아태열대생물의학잡지（영문판）
Asian pacific Journal of Tropical Biomedicine
2015年
12期
987-991
,共5页
Salmonella enterica serovar%typhimurium%sipC gene%TA cloning%Gene construct%pET-32 vector
Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. <br> Methods: In this research, 50 upstream and 30 downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest. <br> Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan-sip C down. <br> Conclusions: The new plasmid (pET-32-sipC up-kan-sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene.
