现代肿瘤医学
現代腫瘤醫學
현대종류의학
Journal of Modern Oncology
2015年
24期
3569-3573
,共5页
MALAT1%小干扰 RNA%侵袭转移%胃癌
MALAT1%小榦擾 RNA%侵襲轉移%胃癌
MALAT1%소간우 RNA%침습전이%위암
MALAT1%small interference RNA%metastasis and invasion%gastric cancer
目的：探讨 LncRNA －MALAT1对缺氧诱导胃癌侵袭转移的影响。方法：RT －PCR 检测胃癌组织标本和胃癌细胞株中 MALAT1的表达；通过基因重组方法构建 MALAT1的 siRNA 载体，并感染人 SGC7901及MKN45胃癌细胞，RT －PCR 验证 siRNA 干扰有效；Transwell 实验研究其对胃癌细胞 SGC7901及 MKN45侵袭转移能力的影响。结果：MALAT1在胃癌组织和胃癌细胞株中的表达高于癌旁组织和胃正常上皮细胞；缺氧能够促进 SGC7901及 MKN45细胞的侵袭转移能力，而下调 MALAT1能够明显抑制缺氧条件下 SGC7901及MKN45细胞的侵袭转移能力。结论：MALAT1在促进胃癌细胞的侵袭转移中发挥重要作用，为胃癌靶向治疗提供理论依据。
目的：探討 LncRNA －MALAT1對缺氧誘導胃癌侵襲轉移的影響。方法：RT －PCR 檢測胃癌組織標本和胃癌細胞株中 MALAT1的錶達；通過基因重組方法構建 MALAT1的 siRNA 載體，併感染人 SGC7901及MKN45胃癌細胞，RT －PCR 驗證 siRNA 榦擾有效；Transwell 實驗研究其對胃癌細胞 SGC7901及 MKN45侵襲轉移能力的影響。結果：MALAT1在胃癌組織和胃癌細胞株中的錶達高于癌徬組織和胃正常上皮細胞；缺氧能夠促進 SGC7901及 MKN45細胞的侵襲轉移能力，而下調 MALAT1能夠明顯抑製缺氧條件下 SGC7901及MKN45細胞的侵襲轉移能力。結論：MALAT1在促進胃癌細胞的侵襲轉移中髮揮重要作用，為胃癌靶嚮治療提供理論依據。
목적：탐토 LncRNA －MALAT1대결양유도위암침습전이적영향。방법：RT －PCR 검측위암조직표본화위암세포주중 MALAT1적표체；통과기인중조방법구건 MALAT1적 siRNA 재체，병감염인 SGC7901급MKN45위암세포，RT －PCR 험증 siRNA 간우유효；Transwell 실험연구기대위암세포 SGC7901급 MKN45침습전이능력적영향。결과：MALAT1재위암조직화위암세포주중적표체고우암방조직화위정상상피세포；결양능구촉진 SGC7901급 MKN45세포적침습전이능력，이하조 MALAT1능구명현억제결양조건하 SGC7901급MKN45세포적침습전이능력。결론：MALAT1재촉진위암세포적침습전이중발휘중요작용，위위암파향치료제공이론의거。
Objective:To explore the effect of LncRNA -MALAT1 on metastasis and invasion in gastric cancer cell SGC7901 and MKN45 under normoxia and hypoxia conditions.Methods:Using Real -time PCR to detect the ex-pression of MALAT1 in gastric cancer tissue and cell lines.Employing the small interfering RNA(siRNA)technique to observe the effect of knockdown of MALAT1 on human gastric cancer cell metastasis and invasion in vitro.Results:MALAT1 was highly expressed in gastric cancer tissues and cell lines compared with adjacent gastric tissues and gas-tric epithelial mucosa cells.Hypoxia significantly increased the migration and invasion potential of SGC7901 and MKN45,which was consistent with previous reports.After transfection with an siRNA to decrease endogenous MAL-AT1 expression,the hypoxia -induced migration and invasion of SGC7901 and MKN45 cells were dramatically dimin-ished.Conclusion:LncRNA -MALAT1 expression is increased in hypoxic GC and promotes hypoxia -induced GC metastasis and invasion.MALAT1 maybe an important target for therapy of metastasis in gastric cancer.
