CAJ | 학술논문

背景：组织工程椎间盘为椎间盘退行性变疾病的生物治疗提供了新的方法，组织工程纤维环是构建组织工程椎间盘的关键之一。目的：从纤维环结构特点、材料支架、种子细胞3方面，归纳总结组织工程纤维环的研究进展。方法：用计算机检索PubMed、万方数据库，限定时间为2000至2015年，中英文检索词分别为“组织工程，椎间盘，纤维环，种子细胞，支架材料，构建”和“tissue engineering, intervertebral disc，annulus fibrosus， seed cel， scaffold， construction”。按纳入和排除标准对文献进行筛选，保留48篇文章进行综述。结果与结论：早期的组织工程纤维环的研究仅侧重于支架上的细胞黏附、细胞增殖和细胞外基质的分泌，目前的组织工程纤维环可以在细胞功能、组织结构和力学特点等方面比较接近于天然纤维环，在动物实验中取得了一定的成果。但是以目前的材料和技术，要构建完全类似于天然纤维环的组织工程纤维环仍具有一定的难度，支架材料、培养条件、种子细胞的获取等诸多方面仍需要进一步完善。目前的构建策略主要集中在单相的纤维环支架上，双相纤维环及完整椎间盘支架将是研究的趋势。干细胞的诱导分化技术为组织工程纤维环提供了广阔的种子细胞来源。
배경：조직공정추간반위추간반퇴행성변질병적생물치료제공료신적방법，조직공정섬유배시구건조직공정추간반적관건지일。목적：종섬유배결구특점、재료지가、충자세포3방면，귀납총결조직공정섬유배적연구진전。방법：용계산궤검색PubMed、만방수거고，한정시간위2000지2015년，중영문검색사분별위“조직공정，추간반，섬유배，충자세포，지가재료，구건”화“tissue engineering, intervertebral disc，annulus fibrosus， seed cel， scaffold， construction”。안납입화배제표준대문헌진행사선，보류48편문장진행종술。결과여결론：조기적조직공정섬유배적연구부측중우지가상적세포점부、세포증식화세포외기질적분비，목전적조직공정섬유배가이재세포공능、조직결구화역학특점등방면비교접근우천연섬유배，재동물실험중취득료일정적성과。단시이목전적재료화기술，요구건완전유사우천연섬유배적조직공정섬유배잉구유일정적난도，지가재료、배양조건、충자세포적획취등제다방면잉수요진일보완선。목전적구건책략주요집중재단상적섬유배지가상，쌍상섬유배급완정추간반지가장시연구적추세。간세포적유도분화기술위조직공정섬유배제공료엄활적충자세포래원。
BACKGROUND:Tissue-engineered intervertebral disc has provided a new biological therapeutic approach for intervertebral disc degeneration. Tissue-engineered annulus fibrosus is one key step of constructing a complete tissue-engineered intervertebral disc. OBJECTIVE:To sum up the research progress of tissue-engineered annulus fibrosus from the folowing aspects: structural features, scaffold materials, seed cels. METHODS:PubMed database and Wanfang database (2000-2015) were retrieved by the key words of “tissue engineering, intervertebral disc, annulus fibrosus, seed cel, scaffold, construction” in Chinese and in English, respectively. According to inclusion and exclusion criteria, 48 literatures were involved for summarization. RESULTS AND CONCLUSION: Previous studies about tissue-engineered annulus fibrosus only focused on cel adhesion, proliferation and extracelular matrix secretion on the scaffold. Currently, tissue-engineered annulus fibrosus exhibit similar features to the natural annulus fibrosus in the folowing aspects: cel function, tissue structure and mechanical features, and relevant animal experiments have achieved certain results in animal experiments. However, it is stil difficult to build a tissue-engineered annulus fibrosus entirely similar to the natural one, and we need to further improve scaffold materials, culture conditions, colection of seed cels. The current strategies of annulus fibrosus construction stil focus on single phase of scaffold, and the biphasic scaffold and complete intervertebral disc scaffold wil be the trend of the researches. Technology of induced differentiation of stem cels provides a broach source of seed cels for tissue-engineered annulus fibrosus.