CAJ | 학술논문

背景：颌面部骨缺损以及牙周组织缺损是影响人类功能和美观的重要疾病之一，组织工程骨成为了修复颌面部骨和牙周组织缺损的主要手段。目前已形成了种子细胞及细胞共培养、支架材料、微环境三者复合以构建组织工程骨的基本模式，将组织工程骨进行预血管化并促进其快速成骨可以减少植入物的坏死、吸收，提升骨缺损修复的成功概率。目的：归纳总结近5年内组织工程骨在口腔颌面部及牙周组织上的研究新进展。方法：对CNKI数据库和PubMed数据库2010年至2015年文献进行检索，中文检索关键词为：颌面；组织工程骨；骨再生；血管化；基因修饰；种子细胞；支架材料；微环境，英文检索词为：oral and maxilofacial， bone tissue engineering， bone regeneration，vascularization，Genetic modification，Seed cels，Support material， Micro environment。排除无关性研究、重复性研究，保留68篇中英文文献进行归纳总结。结果与结论：组织工程骨在修复口腔颌面部及牙周缺损上的应用得到了巨大发展，在三维结构的支架材料上加载基因修饰的种子细胞可以有效的促进血管化进程，提高成骨效应，增加颌骨缺损修复术的成功概率。此外，在牙槽嵴缺损处或新鲜拔牙窝处植入组织工程骨材料，可以有效的恢复和保存牙槽嵴高度和宽度，为后续的修复治疗保证了良好的骨条件。在植入组织工程骨后，可在缺损处加载不同的外部环境刺激，可以通过调节细胞外基质成分或信号通路来改变血管化进程，而血管化是建立有效血循环，保证植入支架成功的前提条件。
배경：합면부골결손이급아주조직결손시영향인류공능화미관적중요질병지일，조직공정골성위료수복합면부골화아주조직결손적주요수단。목전이형성료충자세포급세포공배양、지가재료、미배경삼자복합이구건조직공정골적기본모식，장조직공정골진행예혈관화병촉진기쾌속성골가이감소식입물적배사、흡수，제승골결손수복적성공개솔。목적：귀납총결근5년내조직공정골재구강합면부급아주조직상적연구신진전。방법：대CNKI수거고화PubMed수거고2010년지2015년문헌진행검색，중문검색관건사위：합면；조직공정골；골재생；혈관화；기인수식；충자세포；지가재료；미배경，영문검색사위：oral and maxilofacial， bone tissue engineering， bone regeneration，vascularization，Genetic modification，Seed cels，Support material， Micro environment。배제무관성연구、중복성연구，보류68편중영문문헌진행귀납총결。결과여결론：조직공정골재수복구강합면부급아주결손상적응용득도료거대발전，재삼유결구적지가재료상가재기인수식적충자세포가이유효적촉진혈관화진정，제고성골효응，증가합골결손수복술적성공개솔。차외，재아조척결손처혹신선발아와처식입조직공정골재료，가이유효적회복화보존아조척고도화관도，위후속적수복치료보증료량호적골조건。재식입조직공정골후，가재결손처가재불동적외부배경자격，가이통과조절세포외기질성분혹신호통로래개변혈관화진정，이혈관화시건립유효혈순배，보증식입지가성공적전제조건。
BACKGROUND:Maxilofacial bone and periodontal tissue defect is one of the important diseases that affect human functionality and aesthetic appearance, and bone tissue engineering becomes the main means to repair maxilofacial and periodontal tissue defects. Currently, the basic mode is constructed by the combination of co-culture of seed cels and cels, scaffolds and micro-environment. Pre-vascularization and rapid osteogenesis of tissue-engineered bone can reduce implant necrosis and absorption, and improve repair success rate. OBJECTIVE:To summarize the new progress of bone tissue engineering used in the oral and maxilofacial and periodontal tissue in the past 5 years. METHODS:CNKI database and PubMed database from 2010 to 2015 were searched using the keywords of “oral and maxilofacial, bone tissue engineering, bone regeneration, vascularization, genetic modification, seed cels, support material, microenvironment” in Chinese and English, respectively. After elimination of independent and repetitive studies, 68 articles were included in result analysis. RESULTS AND CONCLUSION: The tissue-engineered bone has achieved tremendous progress in the repair of oral and maxilofacial and periodontal tissue defects. The three-dimensional scaffold with gene-modified seed cels can effectively promote the vascularization, improve the osteogenic effect and increased the probability of success in mandibular defect repair. In addition, tissue-engineered bone implantation into the alveolar ridge defects or fresh extraction fossa can effectively restore and preserve alveolar ridge height and width, to ensure a good bone condition for subsequent restorative treatment. After the implantation of tissue-engineered bone, different external environmental stimuli could be loaded at defect sites, and the extracelular matrix components or signal pathway could be adjusted to change the process of vascularization. Vascularization is a premise condition for the establishment of an effective blood circulation to ensure the success of scaffold implantation.