CAJ | 학술논문

背景：目前越来越多的骨植入材料都采用过氧乙酸-乙醇溶液灭活病毒，但过氧乙酸-乙醇溶液灭活病毒后对脱钙骨基质成骨诱导活性是否有影响，尚没有相关报道。目的：观察过氧乙酸-乙醇溶液灭活病毒后对脱钙骨基质成骨诱导活性的影响。方法：取SD大鼠长管骨制成脱钙骨基质骨粉，将其中一部分骨粉装入明胶胶囊中，采用60Coγ射线辐照灭菌，作为对照组；另一部分骨粉，经过氧乙酸-乙醇溶液处理后装入明胶胶囊中，采用60Coγ射线辐照灭菌，作为实验组。取40只SD大鼠，在其腰椎两侧腰大肌内分别植入实验组与对照组胶囊材料，植入后2，4，6，8周取出所植骨块，进行大体观察及组织学观察。结果与结论：植入后8周，实验组骨块形态不完整，有散在颗粒，光镜下观察可见少量成骨细胞及新生血管；对照组骨块质地较硬，形态完整，光镜下观察可见成熟的骨小粱，骨小梁旁可见成骨细胞包绕及新生的血管，骨小梁间充满脂肪细胞和骨髓细胞。实验组不同时间点的新生微血管计数少于对照组(P <0.001)，实验组植入后8周的钙含量、碱性磷酸酶含量、无机磷含量、新骨生长速率均低于对照组(P<0.001)。表明过氧乙酸-乙醇溶液对脱钙骨基质的成骨诱导能力有一定的负面影响。
배경：목전월래월다적골식입재료도채용과양을산-을순용액멸활병독，단과양을산-을순용액멸활병독후대탈개골기질성골유도활성시부유영향，상몰유상관보도。목적：관찰과양을산-을순용액멸활병독후대탈개골기질성골유도활성적영향。방법：취SD대서장관골제성탈개골기질골분，장기중일부분골분장입명효효낭중，채용60Coγ사선복조멸균，작위대조조；령일부분골분，경과양을산-을순용액처리후장입명효효낭중，채용60Coγ사선복조멸균，작위실험조。취40지SD대서，재기요추량측요대기내분별식입실험조여대조조효낭재료，식입후2，4，6，8주취출소식골괴，진행대체관찰급조직학관찰。결과여결론：식입후8주，실험조골괴형태불완정，유산재과립，광경하관찰가견소량성골세포급신생혈관；대조조골괴질지교경，형태완정，광경하관찰가견성숙적골소량，골소량방가견성골세포포요급신생적혈관，골소량간충만지방세포화골수세포。실험조불동시간점적신생미혈관계수소우대조조(P <0.001)，실험조식입후8주적개함량、감성린산매함량、무궤린함량、신골생장속솔균저우대조조(P<0.001)。표명과양을산-을순용액대탈개골기질적성골유도능력유일정적부면영향。
BACKGROUND:At present, an increasing number of bone graft materials are inactivated using peracetic acid-ethanol solution, but there is no report on whether virus inactivation using peracetic acid-ethanol solution has effects on osteogenic induction of demineralized bone matrix. OBJECTIVE: To explore the effects of virus inactivation using peracetic acid-ethanol solution on the osteogenic activity of demineralized bone matrix. METHODS: Long bones of Sprauge-Dawley rats were selected to make demineralized bone matrix meal. A part of bone meal was placed into gelatin capsules and sterilized by60Coγ irradiation as control group; another part of bone meal was placed into gelatin capsules folowing virus inactivation using peracetic acid-ethanol solution, and then sterilized using60Coγ as experimental group. After that,40 Sprauge-Dawley rats were enroled, and gelatin capsules in the experimental and control groups were respectively implanted into the bilateral psoas muscles of the lumbar spine. At 2, 4, 6, 8 weeks after implantation, the bone grafts were removed for gross and histological observations. RESULTS AND CONCLUSION:At 8 weeks after implantation, the bone mass in the experimental group was not intact in shape with scattered particles, and under light microscope, a few of osteoblasts and new vessels were seen; the bone mass in the control group had hard texture and complete morphology, and under light microscope, mature bone trabeculae wrapped with osteoblasts and new vessels were visible and there were ful of fat cels and bone marrow cells between the bone trabeculae. The number of new microvessels in the experimental group was lower than that in the control group at different time (P < 0.001); at 8 weeks after implantation, the calcium content, alkaline phosphatase content, inorganic phosphorus content and new bone growth rate were all lower in the experimental group than the control group (P < 0.001). These findings indicate that peracetic acid-ethanol for virus inactivation has some negative effects on the osteogenic induction of demineralized bone matrix.