CAJ | 학술논문

目的：利用 RNA 干扰技术沉默人胰腺癌细胞株 PANC －1中 CD44的表达，观察其对肿瘤侵袭性的抑制效果。方法：设计合成有效地干扰 CD44的 shRNA 序列。结果：通过平板克隆形成实验、软琼脂集落形成试验、Transwell 小室侵袭实验发现，胰腺癌细胞株转染干扰 CD44的 shRNA 序列后，其相关的增殖以及侵袭能力明显受抑制。Western Blot 发现随着 CD44的下调，AKT 被逐渐上调，而 p －ERK、p －AKT 却被逐渐下调。结论：靶向 CD44的 shRNA 技术可有效降低人胰腺癌细胞株 PANC －1的侵袭能力，可能的机制与影响其下游的 p －AKT、p －ERK 表达有关。针对 CD44的 RNA 干扰具有潜在的临床价值。
목적：이용 RNA 간우기술침묵인이선암세포주 PANC －1중 CD44적표체，관찰기대종류침습성적억제효과。방법：설계합성유효지간우 CD44적 shRNA 서렬。결과：통과평판극륭형성실험、연경지집락형성시험、Transwell 소실침습실험발현，이선암세포주전염간우 CD44적 shRNA 서렬후，기상관적증식이급침습능력명현수억제。Western Blot 발현수착 CD44적하조，AKT 피축점상조，이 p －ERK、p －AKT 각피축점하조。결론：파향 CD44적 shRNA 기술가유효강저인이선암세포주 PANC －1적침습능력，가능적궤제여영향기하유적 p －AKT、p －ERK 표체유관。침대 CD44적 RNA 간우구유잠재적림상개치。
Objective:We investigated the potential functions of shRNA in CD44 targeting in pancreatic cancer cells.Methods:To devise and synthesis of effectively interference of shRNA sequence of CD44,which was transefect-ed to the pancreatic cancer cells.Results:After the transfection,pancreatic cancer cells'proliferation and invasion a-bilities were obviously inhibited by the colony formation assay,soft agar colony formation test,Transwell chamber inva-sion assay.Western Blot showed that p -ERK,p -AKT was reduced and the AKT were gradually raised in protein levels.Conclusion:shRNA targets CD44 in pancreatic cancer cells and suppresses pancreatic cancer cell growth and metastasis in vitro.This project may provide highly effective targeted therapy for pancreatic cancer and provide a new idea and method for targeting CD44.