山东农业科学
山東農業科學
산동농업과학
Shandong Agricultural Sciences
2015年
11期
120-124
,共5页
冯立娟%尹燕雷%杨雪梅%李英朋
馮立娟%尹燕雷%楊雪梅%李英朋
풍립연%윤연뢰%양설매%리영붕
石榴%高效液相色谱%没食子酸%3 -脱氢莽草酸%五没食子酰葡萄糖
石榴%高效液相色譜%沒食子痠%3 -脫氫莽草痠%五沒食子酰葡萄糖
석류%고효액상색보%몰식자산%3 -탈경망초산%오몰식자선포도당
Pomegranate%High performance liquid chromatography%Gallic acid%3 -dehydroshikimic acid%Pentagalloylglucose
建立了‘泰山三白甜’石榴果实不同部位没食子酸及其合成相关物质的高效液相色谱（HPLC）定性和定量检测方法。色谱条件：色谱柱为 Agilent Zorbax XDB －C18（4．6 mm ×150 mm，5μm），没食子酸以乙腈－2％冰乙酸水溶液为流动相（20∶80），检测波长270 nm；莽草酸和3－脱氢莽草酸以甲醇－1％磷酸水溶液（5∶95）为流动相，检测波长214 nm；五没食子酰葡萄糖以乙腈－0．5％磷酸水溶液（20∶80）为流动相，检测波长275 nm，4种物质均等度洗脱，流速均为0．8 mL／min，柱温均为30℃。结果表明，4种物质在一定线性范围内的峰面积与质量浓度呈良好的线性关系，相关系数均大于0．99，检测限为0．12～0．24 mg／L；加标回收率为98．9％～102．5％，相对标准偏差（RSD）为1．2％～2．2％。‘泰山三白甜’石榴果实不同部位没食子酸、莽草酸、3－脱氢莽草酸和五没食子酰葡萄糖含量存在显著差异，果皮中含量最高，果汁次之，种子中最低。该方法操作简单，灵敏度高，重复性好，可用于没食子酸及其合成相关物质的快速鉴定及含量测定。
建立瞭‘泰山三白甜’石榴果實不同部位沒食子痠及其閤成相關物質的高效液相色譜（HPLC）定性和定量檢測方法。色譜條件：色譜柱為 Agilent Zorbax XDB －C18（4．6 mm ×150 mm，5μm），沒食子痠以乙腈－2％冰乙痠水溶液為流動相（20∶80），檢測波長270 nm；莽草痠和3－脫氫莽草痠以甲醇－1％燐痠水溶液（5∶95）為流動相，檢測波長214 nm；五沒食子酰葡萄糖以乙腈－0．5％燐痠水溶液（20∶80）為流動相，檢測波長275 nm，4種物質均等度洗脫，流速均為0．8 mL／min，柱溫均為30℃。結果錶明，4種物質在一定線性範圍內的峰麵積與質量濃度呈良好的線性關繫，相關繫數均大于0．99，檢測限為0．12～0．24 mg／L；加標迴收率為98．9％～102．5％，相對標準偏差（RSD）為1．2％～2．2％。‘泰山三白甜’石榴果實不同部位沒食子痠、莽草痠、3－脫氫莽草痠和五沒食子酰葡萄糖含量存在顯著差異，果皮中含量最高，果汁次之，種子中最低。該方法操作簡單，靈敏度高，重複性好，可用于沒食子痠及其閤成相關物質的快速鑒定及含量測定。
건립료‘태산삼백첨’석류과실불동부위몰식자산급기합성상관물질적고효액상색보（HPLC）정성화정량검측방법。색보조건：색보주위 Agilent Zorbax XDB －C18（4．6 mm ×150 mm，5μm），몰식자산이을정－2％빙을산수용액위류동상（20∶80），검측파장270 nm；망초산화3－탈경망초산이갑순－1％린산수용액（5∶95）위류동상，검측파장214 nm；오몰식자선포도당이을정－0．5％린산수용액（20∶80）위류동상，검측파장275 nm，4충물질균등도세탈，류속균위0．8 mL／min，주온균위30℃。결과표명，4충물질재일정선성범위내적봉면적여질량농도정량호적선성관계，상관계수균대우0．99，검측한위0．12～0．24 mg／L；가표회수솔위98．9％～102．5％，상대표준편차（RSD）위1．2％～2．2％。‘태산삼백첨’석류과실불동부위몰식자산、망초산、3－탈경망초산화오몰식자선포도당함량존재현저차이，과피중함량최고，과즙차지，충자중최저。해방법조작간단，령민도고，중복성호，가용우몰식자산급기합성상관물질적쾌속감정급함량측정。
The qualitative and quantitative determination methods of gallic acid and its synthetic related substances in different parts of Taishansanbaitian pomegranate fruit were developed by high performance liquid chromatography (HPLC).The chromatographic column was Agilent Zorbax XDB -C1 8 (4.6 mm ×1 50 mm, 5 μm).The mobile phase of gallic acid was acetonitrile and 2% glacial acetic acid solution with the ratio of 20∶80.Its determine wavelength was 270 nm.The mobile phase of shikimic acid and 3 -dehydroshikimic acid was methanol and 1 % phosphoric acid with the ratio of 5∶95.Their determine wavelengths both were 21 4 nm.The mobile phase of pentagalloylglucose was acetonitrile and 0.5% phosphoric acid with the ratio of 20∶80.Its determine wavelength was 275 nm.The 4 substances were all isocratic elution with flow rate of 0.8 mL/min and column temperature of 30℃.The results indicated that the peak area and mass concentration of 4 substances presented good linearities in certain ranges.The correlation coefficients of 4 substances were all o-ver 0.99.The detection limits were 0.1 2 ~0.24 mg/L.The standard recovery rates were 98.9% ~1 02.5%. The relative standard deviationas (RSDs)were 1 .2% ~2.2%.The contents of gallic acid,shikimic acid, 3 -dehydroshikimic acid and pentagalloylglucose were significantly different in different parts of Taishansan-baitian pomegranate fruit.The contents were the highest in fruit peel,followed by juice and seed.The determi-nation method was simple,and had high sensitivity and good reproducibility,which could be used for rapid i-dentification and determination of gallic acid and its synthetic related substances in pomegranate fruit.
