CAJ | 학술논문

目的：比较采用电穿孔和脂质体 lipofectamine 两种方法转染人肝癌细胞的效率优劣。方法：分别采用电穿孔和脂质体转染 miR －218进入人肝癌细胞 HepG2，采用 RT －PCR、Western blot、流式细胞仪、Tunel 检测等方法比较两种方法的转染后对人肝癌细胞的影响。结果：采用两种方法都可以成功将 miR －218转染进人肝癌细胞 HepG2中，降低了 HepG2中 miR －218靶基因 RNA 编辑酶 ADAR1的表达，使得细胞凋亡增加，但电穿孔转染组效率更高。结论：电穿孔转染是一种安全、高效的转染方法，值得推广。
목적：비교채용전천공화지질체 lipofectamine 량충방법전염인간암세포적효솔우렬。방법：분별채용전천공화지질체전염 miR －218진입인간암세포 HepG2，채용 RT －PCR、Western blot、류식세포의、Tunel 검측등방법비교량충방법적전염후대인간암세포적영향。결과：채용량충방법도가이성공장 miR －218전염진인간암세포 HepG2중，강저료 HepG2중 miR －218파기인 RNA 편집매 ADAR1적표체，사득세포조망증가，단전천공전염조효솔경고。결론：전천공전염시일충안전、고효적전염방법，치득추엄。
Objective:To compare the transfection efficiencies of miR -218 into human hepatocarcinoma cells HepG2 by electroporation or lipofectamine.Methods:miR -218 was transfected either by electroporation or by lipo-fectamine.Cells were collected,and then the condition of HepG2 cells were detected by RT -PCR,Western blot,Flow Cytometry and Tunel test.Results:Both two methods could transfect miR -218 into HepG2 swimmingly,leading to the increase of apoptosis,with the apporach of electroporation had abetter effect.Conclusion:Electroporation transfection is a safe,effective approach,which is worthy promotion.