CAJ | 학술논문

研究发现锌指（Zinc－finger HIT，Zf－HIT）蛋白在植物的抗逆性方面有重要的调控作用。本研究从普通小麦中克隆到一个含有 PAPA －1－like[Pim －1－associated protein －1（PAP －1）－associated protein －1－like]和 Zf －HIT 结构域的蛋白质基因，命名为 TaPAPA1。通过测序和序列分析，得到三条 gDNA 和三条cDNA 序列，均包含6个外显子和5个内含子。根据序列间的差异位点设计基因组特异引物，用中国春小麦缺体—四体系将该基因定位在小麦第五同源群。TaPAPA1－5A、TaPAPA1－5B 和 TaPAPA1－5D 的编码序列长度分别为1473、1473 bp 和1470 bp，分别编码490、490和489个氨基酸。在5B 染色体检测到该基因的一个 SNP 位点，并开发 AS －PCR 标记 TaPAPA1－5B －AS1／2。利用 RIL（Recombinant inbred line）群体，把该标记定位在 D －1207571和 D －1100783之间，遗传距离分别是0．51 cM和0．71 cM。
연구발현자지（Zinc－finger HIT，Zf－HIT）단백재식물적항역성방면유중요적조공작용。본연구종보통소맥중극륭도일개함유 PAPA －1－like[Pim －1－associated protein －1（PAP －1）－associated protein －1－like]화 Zf －HIT 결구역적단백질기인，명명위 TaPAPA1。통과측서화서렬분석，득도삼조 gDNA 화삼조cDNA 서렬，균포함6개외현자화5개내함자。근거서렬간적차이위점설계기인조특이인물，용중국춘소맥결체—사체계장해기인정위재소맥제오동원군。TaPAPA1－5A、TaPAPA1－5B 화 TaPAPA1－5D 적편마서렬장도분별위1473、1473 bp 화1470 bp，분별편마490、490화489개안기산。재5B 염색체검측도해기인적일개 SNP 위점，병개발 AS －PCR 표기 TaPAPA1－5B －AS1／2。이용 RIL（Recombinant inbred line）군체，파해표기정위재 D －1207571화 D －1100783지간，유전거리분별시0．51 cM화0．71 cM。
Zinc -finger HIT protein plays an important role in the regulation of plant defense and stress response.In this study,a gene named TaPAPA1,who encoded one protein containing PAPA -1 -like [Pim -1 -associated protein -1 (PAP -1 )-associated protein -1 -like]and Zf -HIT (Zinc -finger HIT)domain, was cloned from wheat.Through sequence analysis,three cDNA and three gDNA sequences were got,which all had six exons and five introns.According to different sites among three gDNA sequences,three pairs of genome special primers were designed,and these genes were located in homologous group 5 using nullisomic -tetrasomic lines of Chinese Spring.The coding sequences of TaPAPA1 -5A,TaPAPA1 -5B and TaPAPA1 -5D were 1 473,1 473 bp and 1 470 bp,which encoded polypepetides with 490,490 and 489 amino acids,respectively. According to the SNP locus in TaPAPA1 -5B,the AS -PCR marker TaPAPA1 -5B -AS1 /2 was developed.U-sing a RIL (recombinant inbred line)population,the marker was located between marker D -1207571 and D -1100783 on 5B by linkage analysis with the genetic distance of 0.51 cMand 0.71 cM,respectively.