中国临床新医学
中國臨床新醫學
중국림상신의학
Chinese Journal of New Clinical Medicine
2015年
11期
1013-1015
,共3页
蒋丽君%刘建红%宋玮%潘凤锦%全志豪%莫晓云%雷志坚%梁碧芳%唐华林%谢宇萍%龙胜泽%吴思旸
蔣麗君%劉建紅%宋瑋%潘鳳錦%全誌豪%莫曉雲%雷誌堅%樑碧芳%唐華林%謝宇萍%龍勝澤%吳思旸
장려군%류건홍%송위%반봉금%전지호%막효운%뢰지견%량벽방%당화림%사우평%룡성택%오사양
间歇低氧%高血压%SD大鼠
間歇低氧%高血壓%SD大鼠
간헐저양%고혈압%SD대서
Intermittent hypoxia%Hypertension%Sprague-Dawley rats
目的：建立慢性间歇低氧并高血压SD大鼠模型，为研究阻塞性睡眠呼吸暂停低通气综合征（OSAHS ）并高血压的发病机制提供简单可复制的动物模型。方法氧舱系统分为控制系统、气源系统、暴露舱三个部分。选8～10周龄SD雄性大鼠24只（200～250 g）随机分成间歇低氧干预组和对照组。间歇低氧干预组12只暴露于间歇低氧舱内，白天给予间歇低氧干预（6％～8％的 O240 s／21％的O280 s，如此往复循环8 h）；对照组12只暴露于常氧舱。在安静状态下检测大鼠尾动脉收缩压，每只连续测量3次，取平均值。结果实验第35天，间歇低氧干预组尾动脉收缩压[（136．21±32．46）mmHg]高于对照组[（121．37±35．37）mmHg]（P ＜0．05）。结论控制精确气源通断时间，可有效地模拟间歇低氧过程，重复性佳，是研究间歇低氧所致高血压机制的理想实验模型。
目的：建立慢性間歇低氧併高血壓SD大鼠模型，為研究阻塞性睡眠呼吸暫停低通氣綜閤徵（OSAHS ）併高血壓的髮病機製提供簡單可複製的動物模型。方法氧艙繫統分為控製繫統、氣源繫統、暴露艙三箇部分。選8～10週齡SD雄性大鼠24隻（200～250 g）隨機分成間歇低氧榦預組和對照組。間歇低氧榦預組12隻暴露于間歇低氧艙內，白天給予間歇低氧榦預（6％～8％的 O240 s／21％的O280 s，如此往複循環8 h）；對照組12隻暴露于常氧艙。在安靜狀態下檢測大鼠尾動脈收縮壓，每隻連續測量3次，取平均值。結果實驗第35天，間歇低氧榦預組尾動脈收縮壓[（136．21±32．46）mmHg]高于對照組[（121．37±35．37）mmHg]（P ＜0．05）。結論控製精確氣源通斷時間，可有效地模擬間歇低氧過程，重複性佳，是研究間歇低氧所緻高血壓機製的理想實驗模型。
목적：건립만성간헐저양병고혈압SD대서모형，위연구조새성수면호흡잠정저통기종합정（OSAHS ）병고혈압적발병궤제제공간단가복제적동물모형。방법양창계통분위공제계통、기원계통、폭로창삼개부분。선8～10주령SD웅성대서24지（200～250 g）수궤분성간헐저양간예조화대조조。간헐저양간예조12지폭로우간헐저양창내，백천급여간헐저양간예（6％～8％적 O240 s／21％적O280 s，여차왕복순배8 h）；대조조12지폭로우상양창。재안정상태하검측대서미동맥수축압，매지련속측량3차，취평균치。결과실험제35천，간헐저양간예조미동맥수축압[（136．21±32．46）mmHg]고우대조조[（121．37±35．37）mmHg]（P ＜0．05）。결론공제정학기원통단시간，가유효지모의간헐저양과정，중복성가，시연구간헐저양소치고혈압궤제적이상실험모형。
Objective To establish a hypertension rat model induced by chronic intermittent hypoxia, and to provide a simple and duplicate animal model for the study of the pathogenesis of hypertension in OSAHS.Methods The intermittent hypoxia system was used to conduct the experiment , including the control subsystem, the gas supply subsystem and the exposed cabin.24 male Sprague-Dawley rats, aged 8 to 10 weeks, and weighted from 200 to 250 g were randomly divided into two groups:the intermittent hypoxia intervention group and the control group.The inter-mittent hypoxia intervention group(n=12) was exposed to 6%to 8%oxygen for 40 seconds and then to 21 %oxy-gen for 80 seconds in the exposed cabin for 8 hours per day with many intermittent hypoxia cycles.The control group ( n=12) was exposed to certain concentration oxygen chamber.The tail artery systolic pressure of the rats was taken at rest states for three consecutive times and the average was calculated.Results The intermittent hypoxia interven-tion group had a significantly higher systolic blood pressure than the control group at the 35th day of the experiment [(136.21 ±32.46)mmHg vs (121.37 ±35.37)mmHg, P<0.05].Conclusion Precisely aerating the oxygen on and off in an certain range of concentration can effectively make a simulated intermittent hypoxia process, which is an ideal animal model for studying the mechanisms of intermittent hypoxia-induced hypertension.
