CAJ | 학술논문

为探讨外源ABA对低温胁迫下冬小麦膜质脂肪酸的影响，以强抗寒品种东农冬麦1号和冷敏感品种济麦22为材料，三叶期叶面喷施适宜浓度ABA，大田自然降温条件下，分别于10 d最低温平均4、0、-10、-25℃时取样叶片和分蘖节，测定膜质脂肪酸的含量变化。结果表明，叶片中，两种小麦不饱和指数均呈逐渐下降趋势，其中东农冬麦1号不饱和脂肪酸含量低于济麦22；分蘖节中，东农冬麦1号亚麻酸含量在降温过程中逐渐升高，而棕榈酸含量始终低于济麦22，说明亚麻酸和棕榈酸与冬小麦抗寒性关系密切。外源ABA处理后，两种小麦叶片和分蘖节中的不饱和脂肪酸含量均较未处理高，且东农冬麦1号叶片不饱和指数在零上温度时均大于济麦22，叶片和分蘖节分别于4℃、-25℃达到最大值，其中东农冬麦1号分蘖节不饱和脂肪酸呈逐渐上升趋势、且不饱和指数均高于济麦22，达到差异极显著。外源ABA使两品种小麦电导率、均有不同程度降低，东农冬麦1号MDA降幅大于济麦22，且达到差异显著，表明ABA对强抗寒品种膜脂抗寒性的促进作用大于冷敏感品种。
위탐토외원ABA대저온협박하동소맥막질지방산적영향，이강항한품충동농동맥1호화랭민감품충제맥22위재료，삼협기협면분시괄의농도ABA，대전자연강온조건하，분별우10 d최저온평균4、0、-10、-25℃시취양협편화분얼절，측정막질지방산적함량변화。결과표명，협편중，량충소맥불포화지수균정축점하강추세，기중동농동맥1호불포화지방산함량저우제맥22；분얼절중，동농동맥1호아마산함량재강온과정중축점승고，이종려산함량시종저우제맥22，설명아마산화종려산여동소맥항한성관계밀절。외원ABA처리후，량충소맥협편화분얼절중적불포화지방산함량균교미처리고，차동농동맥1호협편불포화지수재령상온도시균대우제맥22，협편화분얼절분별우4℃、-25℃체도최대치，기중동농동맥1호분얼절불포화지방산정축점상승추세、차불포화지수균고우제맥22，체도차이겁현저。외원ABA사량품충소맥전도솔、균유불동정도강저，동농동맥1호MDA강폭대우제맥22，차체도차이현저，표명ABA대강항한품충막지항한성적촉진작용대우랭민감품충。
Two winter wheat cultivars of Dongnongdongmai1(freezing-tolerant) and Jimai 22 (freezing-sensitive) were used to investigate the effects of exogenous ABA on lipid composition under cold stress. Leaves were sprayed with 10-5 mol·L-1 ABA when seedings were at the 3-trure-leaf stage in the lowest temperature for 10 d at 4, 0,-10,-25℃ to study the effect of exogenous ABA on lipid composition. The results showed that the contents of unsaturated fatty acids gradually decresed in the two cultivars, and the IUFA of Dongnongdongmai 1 were lower than Jimai 22 in leaves. The linolenic acid contents of Dongnongdongmai 1 was higher and the palmitic acid contents was lower that those of Jimai 22, C(16??1) and C(18??3) were determined as the reference for freezing rolerant indicators. The treatment under exogenous ABA have highter unsaturated fatty acids contents than the treatment of water. especial y in maxmum temperature of 4℃,-25℃in leaves and nods.The unsaturated fatty acids gradual y incresed on the nods of Dongnongdongmai 1, and the IUFA was higher than Jimai 22, achieved very significant levels. The contents of MDA and ralative electrical conductivity gradual y decresed in the two cultivars, and the decreased in Dongnongdongmai 1 were lower than Jimai 22, and achieved significant levels. These showed that ABA improving cold hardiness of freezing-tolerant culrivar than freezing-sensitive cultivar.